传染性腔上囊病病毒广西流行株免疫原性的研究  

Immunogenicity of prevalent infectious bursal disease virus strains in Guangxi,China

作　　者：黄志永[1,2] 韦平[1] 吴俊姬[1] 陆有飞[1] 李葆泓[1] 钟雅婷[1] 阳秀英[1] 何秀苗[3] 

机构地区：[1]广西大学养禽与禽病研究所,广西南宁530005 [2]广西融安县水产畜牧兽医局,广西融安545400 [3]广西民族大学,广西南宁530006

出　　处：《中国兽医科学》2009年第10期880-885,共6页Chinese Veterinary Science

基　　金：广西科技攻关项目(2007A04024;0537008-3A;0719004-3D);教育部科研重点项目(02116);广西自然科学基金项目(桂科自0728058)

摘　　要：分别制备传染性腔上囊病病毒（IBDV）广西流行毒株040124、BH11、TSC-2（9）和YL052以及参考强毒株CJ801-BKF的灭活油乳剂疫苗（OEV）及其多价OEV,分别进行IBD商品疫苗毒株B87（in）和FW2512对4个广西流行毒株的攻毒免疫保护试验、流行毒株间的免疫交叉保护试验、多价OEV不同免疫剂量及其与CJ801-BKF株OEV的免疫保护对比试验。结果显示,2个IBD商品疫苗均不能对4个流行毒株提供完全的保护;4个流行毒株的OEV对同源毒株的攻毒均可产生100%保护,其中040124株OEV的交叉免疫保护性最好;在毒株免疫组与未免疫组免疫器官指数的比较中发现,TSC-2（9）株OEV免疫鸡的免疫器官受病毒的损伤程度最严重,而040124、BH11和YL052株OEV的免疫均能较好地保护鸡的免疫器官免受病毒的损伤;多价OEV 3个免疫剂量的免疫保护指数（IPI）为80%~100%,1 mL免疫组的IPI显著高于0.25 mL免疫组（P〈0.05）,而1 mL免疫组与0.5 mL免疫组差异不显著（P〉0.05）;多价OEV的免疫效力显著高于毒株YL052、TSC-2（9）和CJ801-BKF的OEV（P〈0.05）。结果表明,本研究制备的多价灭活疫苗适用于本地IBD的防制。The immune protection tests of the infectious bursal disease （IBD） commercial vaccine strains B87（in） and FW2512 against the four Guangxi isolates 040124,BH11,TSC-2（9） and YL052,the cross-protective immunity tests among the four isolates, the protection contrast tests of the multivalent inactivated oil emulsion vaecine（OEV） from isolates 040124,BH11 and TSC 2（9） at different immune dosages and a protection contrast test of the multivalent inactivated OEV with the OEV developed from the reference virulent strain CJS01-BKF were conducted respectively. The results showed that the two commercial vaccines could not provide full protection against the four field isolates, while 100% protections were obtained with the OEVs of the four isolates against the homogeneous viruses,and the OEV of 040124 provided the highest cross-protection. Birds vaccinated with OEVs of isolate TSC-2（9） had the lowest protection of immune organs against the challenge of isolates by having significant difference in immune organs index （IOI） compared with the un-infected control birds（P〈0.05）, while the birds vaccinated with OEVs of isolates 040124,BHll and YL052 had no significant difference in IOI compared with the un-infected control birds（P〉0.05）. Immune protection index（IPI） of the multivalent OEV with 3 different immune dosages of 1 mL,0.5 mL and 0.25 mL was from 80% to 100%,per bird dosages 1 mL had significant higher IPI than that of dosage 0.25 mL（P〈0.05） ,while dosages 1 mL and 0.5 mL had no significant difference on IPI（P〉0.05）. These results demonstrated that the immune efficacy of multivalent OEV was the best among all the OEVs tested,which might be used for the prevention and control of IBD in Guangxi.

关 键 词：传染性腔上囊病病毒 免疫原性 油乳剂疫苗 最佳免疫剂量 免疫效力 

分 类 号：S852.659.4[农业科学—基础兽医学]