APP/PS1转基因鼠的骨微结构及骨密度分析  

To analyse the bone microarchitecture and bone mineral density of APP/PS1 transgenic mice

作　　者：王铜浩[1] 杨茂伟[1] 赵梦楠[1] 初立伟[1] 

机构地区：[1]中国医科大学附属第一医院骨科

出　　处：《中国骨质疏松杂志》2009年第9期632-636,共5页Chinese Journal of Osteoporosis

摘　　要：下载PDF阅读器目的 应用高分辨率CT（Micro-CT）定量研究APP/PS1转基因鼠（老年痴呆鼠模型）胫骨近端骨微结构及骨密度的变化.方法 3月龄雌性APP/PS1转基因鼠（n=6）和野生型小鼠（n=6）自由摄食和饮水,分别于12月龄时处死,取其胫骨,应用Micro-CT进行骨微结构及骨密度分析.结果 APP/PS1转基因组小鼠胫骨近端骨微结构、骨密度参数明显小于野生型小鼠（P〈0.05）;Micro-CT扫描图像显示APP/PS1转基因小鼠的骨小梁数目、骨小梁厚度,骨皮质厚度明显小于野生型小鼠.结论 APP/PS1转基因组小鼠胫骨近端骨微结构、骨密度参数与野生型小鼠相比存在明显的差异, APP/PS1转基因组小鼠更易患骨质疏松.Objective To quantitatively investigate the changes of bone microarchitecture and bone mineral density in proximal tibia of APP/PS1 transgenic mice （a mice model of senile dementia） by micro computer tomography. Methods 3-month-old APP/PS1 transgenic and wild-type female mice （ n = 6/group） were housed with food and tap water available ad libitum. Twelve mice were sacrifced at 12 months of age and the tibias were harvested to analyse the bone microarchitecture and bone mineral density by Micro-CT. Results Bone microarchitecture and bone mineral density in Micro-CT analysis of APP/PS1 transgenic mice were significantly lower than those of wild-type mice（ P 〈 0.05） ; the Micro-CT image of trabecular number, trabecular thickness and conical thickness in APP/PSI transgenic mice were significantly lower than those in wild-type mice. Conclusion Bone microarchitecture and bone mineral density were significantly diverse in APP/PS1 transgenic mice as compared to wild-type mice,APP/PS1 transgenic mice were liable to osteoporosis.

关 键 词：APP/PS1转基因鼠 野生型小鼠 骨质疏松 micro—CT 骨密度 

分 类 号：R681[医药卫生—骨科学]