叶籽银杏GbMADS5基因的克隆与序列分析  

Cloning and Sequence Analysis of GbMADS5 Gene from Ginkgo biloba var.epiphylla Mak.

作　　者：王京梅[1] 李际红[1] 邢世岩[1] 姚林梅[1] 王邑刚 

机构地区：[1]山东农业大学林学院,泰安271018 [2]德州市临邑县林业局,临邑251500

出　　处：《分子植物育种》2009年第5期1015-1020,共6页Molecular Plant Breeding

基　　金：国家自然科学基金资助项目(30671707;30872040)共同资助

摘　　要：MADS-box基因是一类编码转录因子的基因大家族,在植物的发育过程中具有重要作用。本研究采用RT-PCR技术从叶籽银杏(Ginkgo biloba var.epiphylla Mak.)中克隆了MADS-box基因GbMADS5。序列分析表明,该基因编码区全长为666bp,含有一个完整的开放阅读框,编码221个氨基酸残基组成的蛋白质,具有典型的植物MADS-box基因结构,编码肽链包含了MADS区、I区、K区和C末端,但该基因不具有拟南芥(Arabidopsis thaliana)AG基因的N端区域。GbMADS5基因编码的蛋白序列与其它植物的MADS-box蛋白序列有着较高的一致性,与裸子植物(Gymnospermae)的AG基因相似性最高,其中与苏铁(Cycas revolute)的同源性高达91.07%。系统发育树分析显示,GbMADS5基因属于AG亚家族基因。As a kind of transcription factors gene family, MADS-box genes play an important role in plant development processes. MADS-box gene GbMA DS5 was isolated from Ginkgo biloba var. epiphylla Mak. by RT-PCR. Sequence analysis indicated that its coding region was 666 bp, with an open reading flame encoding a 221 amino acids protein, which displayed the structure of typical plant MADS-box gene including MADS domain, I domain, K domain and C terminus. But this gene did not have N terminal ofAG gene from Arabidopsis thaliana. The protein of GbMA DS5 was highly homologous to other MADS-box protein from different species, especially with AG gene from Gymnospermae. The identity of GbMADS5 and AG from Cycas revolute revolute was up to 91.07%. The phylogenetic tree analysis revealed that GbMA DS5 gene belonged to AG subfamily.

关 键 词：叶籽银杏 GbMADS5 MADS-BOX基因 序列分析 

分 类 号：S792.95[农业科学—林木遗传育种]