水稻愈伤组织对不同培养基的适应性、潮霉素的敏感性及其在遗传转化中的利用  

The Adaptability of Rice Callus to Different Media,the Sensitivity to Hygromycin and Utilization in Rice Genetic Transformation

作　　者：康海岐[1] 申国安[2] 

机构地区：[1]四川省农业科学院作物研究所,成都610066 [2]复旦大学遗传所,上海200433

出　　处：《中国生物工程杂志》2009年第10期50-54,共5页China Biotechnology

基　　金：四川省农科院青年基金;四川省财政育种专项(2006YZGG04-009)资助项目

摘　　要：取粳稻(Oryza sativaL.japonica)品种中花11号开花授粉后15天的幼胚诱导愈伤组织,在N6D、N6B5D、MsD和B5D 4种培养基上比较愈伤组织生长情况,证明3种培养基均可以用于水稻愈伤组织的培养,但B5D的效果不如另3种培养基,N6D和N6B5D的培养效果优于Ms培养基,N6B5D的效果略好于N6D。后续实验主要采用N6D培养基。进一步对愈伤组织的HygB敏感性试验,发现30mg/L的HygB对愈伤组织生长有明显的抑制作用,50mg/L的浓度对愈伤的生长抑制作用更彻底,因此认为采用50mg/L的浓度进行抗HygB的愈伤组织筛选比较可靠。利用上述结果,在水稻遗传转化方面进行了应用和验证,发现效果比较明显。The embryo of japonica cultivar Zhonghua 11（ZH11） after flowering fifteen days was used for the callus inducing.This embryogenic callus was inoculated onto four kinds of mediums N6D,N6B5D,MsD and B5D and compared.It suggested that all of the four kinds of mediums could be used in the inducing and culturing of rice callus,but N6B5D was more appropriate than N6D,N6D and N6B5D were better than MsD,MsD was better than B5D.The N6D medium was used to the sensitivity testing of embryogenic callus to Hygromycin concertration.Embryogenic callus was sensitive to the concertration of 30 mg/L,but was thoroughly restrained from the concertration of 50 mg/L.This concertration was suggested to be used in the screening of embryogenic callus resistant to Hygromycin.Using above results,it was validated in rice genetic transformation.

关 键 词：水稻 愈伤组织 培养基 潮霉素 

分 类 号：Q942.5[生物学—植物学]