DNA改组的最新动态及应用前景  

Research on the Latest Developments and Application of DNA Shuffling

作　　者：单世平[1] 夏立秋[1] 丁学知[1] 刘石泉[1,2] 张友明[1] 

机构地区：[1]湖南师范大学生命科学学院微生物分子生物学湖南省重点实验室,长沙410081 [2]湖南城市学院化学与环境工程系,益阳413000

出　　处：《中国生物工程杂志》2009年第10期92-97,共6页China Biotechnology

基　　金：国家"863计划"(2006AA02Z187;2006AA10A212);国家自然科学基金(30670052);国家博士点基金(20060542006)资助项目

摘　　要：DNA改组(DNA shuffling)是目前最方便、有效的一种分子水平的体外定向进化技术,该技术同倾向错误PCR(Error-prone PCR)相结合,通过对单基因或相关基因家族的靶序列进行多轮随机诱变、重组和高通量的筛选,可以有效富集正突变,去除负突变,提高突变文库的丰度,创造新基因和获得期望功能的蛋白质。DNA改组技术已在新药物等领域取得了广泛的应用,极大地推动了现代生物科学和生物技术的发展。该技术同计算机强大的数据分析系统相结合,将会为后基因组学的发展提供强有力的技术平台。DNA shuffling is the most convenient and effective strategies for directed molecular evolution.Together with erro-prone PCR,DNA family shuffling and single-gene DNA shuffling,through iterations of random mutations,recombination and high-throughout screening,can accumulate beneficial mutations while simultaneously removing deleterious mutations,increase the abundance of mutant libraries,create novel genes and obtain desirable functional proteins.Furthermore,DNA shuffling procure broad application in many aspects,especially in pharmaceutical production,and drive the development of biological sciences and biotechnology.Moreover,taking advantage of the tremendous data analysis power,DNA shuffling as a postgenomics technology platform is being increasingly recognized.

关 键 词：DNA改组 倾向错误PCR 基因家族 后基因组学 

分 类 号：Q819[生物学—生物工程]