神经生长因子在脊髓高位损伤大鼠膀胱中表达量的变化与意义  

The Change and Meaning of Nerve Growth Factor in Bladder of Hight-level-spinal Cord Injured Rats

作　　者：刘孝东[1] 何育霖[1] 申吉泓[1] 王黎[1] 张林[2] 张建华[1] 

机构地区：[1]昆明医学院第一附属医院泌尿外科,云南昆明650032 [2]昆明医学院第一附属医院病理科,云南昆明650032

出　　处：《昆明医学院学报》2009年第10期37-41,共5页Journal of Kunming Medical College

摘　　要：目的探讨脊髓高位损伤致逼尿肌反射亢进(detrusor hyperreflexia,DH)大鼠膀胱上神经生长因子(nerve growth factor,NGF)表达量的变化和组织学改变及其与膀胱逼尿肌反射亢进的关系.方法26只SD雌性大鼠分实验组16只,正常对照组10只.实验组于第10胸椎剪断脊髓,4周后作膀胱尿动力学检测,筛选出脊髓损伤伴DH大鼠,用免疫组化法测定大鼠膀胱上NGF的表达量,HE和Masson染色观察膀胱组织学变化.结果脊髓高位损伤大鼠模型实验组16只,死亡4只,产生DH的12只(100%).NGF在两组大鼠膀胱的上皮和逼尿肌上均有表达,上皮表达在包膜,逼尿肌表达在包浆.实验组DH大鼠膀胱的上皮和逼尿肌NGF平均光密度值(0.358±0.011),(0.217±0.011)显著高于正常对照组大鼠膀胱上皮和逼尿肌NGF平均光密度值(0.183±0.012,0.058±0.006)(P<0.05).HE和Massom染色显示脊髓高位损伤后DH大鼠膀胱的逼尿肌和移行上皮均增生.结论脊髓高位损伤后的DH症状与膀胱上皮和逼尿肌上的NGF表达量升高密切相关,可能是其产生的分子生物学基础之一,同时组织学也发生改变.Objective To investigate the change of nerve growth factor in detrusor hyperreflexia bladder of hight-level-spinalized rats and to search for the relationship of NGF with detrusor instable bladder. At the same time, to investigate the changes of histology. ,Methods 26 rats were divide into experimental group （16 rats）, and control group （10 rats）. Rats in experimental group were sheared off spinal cord at the level of 10th thoracie vertebra, after 4 weeks, cystometry was performed and got the rats with detrusor hyperreflexia bladder. Then we detected the level of NGF in detrusor hyperreflexia bladder by immunohistochemieal analysis and compared with which in normal rats bladders. Meanwhile HE and masson dye was used to investigate the changes of histology. Results 16 rats were sheared off spinal cord, 4 rats died after that, 12 rats got detrusor hyperreflexia bladders （100%）.NGF expressed in all rats＇ membrane of epithelium and cytoplasm of detrusor muscle in bladders. The NGF＇ average optical density of-epithelium and detrusor muscle in detrusor hyperreflexia rats （0.358 ± 0.011, 0.217±0.011） were higher than those in control group （0.183±0.012, .0.058±0.006） （P〈0.05）. Gonclusions There is a significant relationship between detrusor hyperreflexiabladder after hight-level-spinal cord injur and the increase of NGF in bladder. The change maybe the molecular biologic base of detrusor ins＇table bladder. Simultaneously the histology changes a lot.

关 键 词：膀胱 逼尿肌反射亢进 神经生长因子 尿动力学 

分 类 号：R694.5[医药卫生—泌尿科学]