车前子多糖抑制氧化型低密度脂蛋白诱导的血管平滑肌细胞增殖及其机制  

Inhibition and Mechanism of Plantain Seed Polysaccharide on Proliferation of Vascular Smooth Muscle Cells Induced by Oxidized Low Density Lipoprotein

作　　者：张宁[1] 王素敏[1] 车文文[1] 张然[1,2] 郭贇 徐斯佳[1] 

机构地区：[1]河北医科大学药理学教研室,石家庄050017 [2]河北省沧州医学高等专科学校,沧州061001

出　　处：《细胞生物学杂志》2009年第5期683-688,共6页Chinese Journal of Cell Biology

基　　金：河北省自然基金资助项目(No.C2007000822)~~

摘　　要：采用组织贴壁培养法建立氧化型低密度脂蛋白(ox-LDL)诱导血管平滑肌细胞(VSMC)的增殖模型,以四甲基偶氮唑蓝(MTT)法观察车前子多糖(plantain seed polysaccharide,PSP)对VSMC增殖的影响,结果表明ox-LDL诱导的VSMC增殖明显(P<0.05),认为该增殖模型成功建立。利用比色法测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、一氧化氮(NO)含量以及一氧化氮合酶(NOS)的活性,探讨PSP在细胞水平的抗氧化作用,结果显示给予PSP后,MDA含量显著下降,SOD、NO和NOS水平明显升高(P<0.05),表明PSP具有一定的抗氧化作用。RT-PCR检测原癌基因(c-myc)和单核细胞趋化蛋白-1(MCP-1)水平,观察PSP对c-myc和MCP-1mRNA表达的影响,以寻找药物的靶点并探讨PSP抗动脉粥样硬化作用的分子机制。结果表明,PSP下调c-mycmRNA和MCP-1mRNA的表达,可能是抗动脉粥样硬化的机制之一。The proliferation model of vascular smooth muscle cells （VSMC） induced by oxidized low density lipoprotein （ox-LDL） was established with the method of tissue explants adherent. MTT assay was used to measure the proliferation of VSMC. The results shows that cell proliferation obviously increased and the prolifera- tion model was successfully established. The content of malondialdehyde （MDA） and nitric oxide （NO）, the activity of superoxide dismutase （SOD） and nitric oxide synthase （NOS） in supernatant were tested by colorimetric method to observe the antioxidation effects of plantain seed polysaccharide （PSP）. Different concentrations of PSP decreased the MDA content, and increased the SOD, NO and NOS activity （P〈0.05）. It indicated that PSP had certain antioxidant effect. RT-PCR was used to detect the expression of c-myc and MCP-1 mRNA to select drug targets and explore the molecular mechanism of the anti-atherosclerosis of PSP. PSP had the anti-atherosclerosis, which is related to the down-regulated expression of c-myc and MCP-1.

关 键 词：车前子多糖 氧化型低密度脂蛋白 血管平滑肌细胞 C-MYC 单核细胞趋化蛋白-1 

分 类 号：R285[医药卫生—中药学]