The proteomic analysis of human neonatal umbilical cord serum by mass spectrometry  

作　　者：Hong-juan SONG Ping ZHANG Xue-jiang GUO Lian-ming LIAO Zuo-min ZHOU Jia-hao SHA Yu-gui CUI HuiJI Jia-yin LIU 

机构地区：[1]China Pharmaceutical University, Nanjing 210009, China [2]Jiangsu Province Laboratory of Reproductive Medicine, Nanjing 210000, China [3]Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210036, China

出　　处：《Acta Pharmacologica Sinica》2009年第11期1550-1558,共9页中国药理学报（英文版）

摘　　要：Aim： To investigate the proteome composition and function of human neonatal arterial umbilical cord. Methods： Serum proteomic analyses were performed on samples from both males and females by using a combination of techniques： （：2） removal of six high-abundance proteins, （2） tryptic digestion of low-abundance proteins, （3） separation of peptide mixtures by reverse-phase high-performance liquid chromatography （RP-HPLC）, and （4） peptide identification using electrospray ionization tandem mass spectrometry （ESI-MS/MS）. Results： A total of 837 non-redundant proteins were identified, with 213 male-specific and 239 female-specific proteins. Among them, 329 proteins were identified by at least 2 distinct peptides. The subcellular localization, function, and pathway involvement for each of the identified proteins were analyzed. A comparison of this neonatal proteorne to that of adult serum proteome revealed novel biomarkers, such as alpha-fetoprotein and periostin that were specific to newborn infants. Conclusion： These data will contribute to a better understanding of the composition of umbilical cord serum and aid the discovery of novel biomarkers for the prenatal diagnosis of fetal abnormalities.Aim： To investigate the proteome composition and function of human neonatal arterial umbilical cord. Methods： Serum proteomic analyses were performed on samples from both males and females by using a combination of techniques： （：2） removal of six high-abundance proteins, （2） tryptic digestion of low-abundance proteins, （3） separation of peptide mixtures by reverse-phase high-performance liquid chromatography （RP-HPLC）, and （4） peptide identification using electrospray ionization tandem mass spectrometry （ESI-MS/MS）. Results： A total of 837 non-redundant proteins were identified, with 213 male-specific and 239 female-specific proteins. Among them, 329 proteins were identified by at least 2 distinct peptides. The subcellular localization, function, and pathway involvement for each of the identified proteins were analyzed. A comparison of this neonatal proteorne to that of adult serum proteome revealed novel biomarkers, such as alpha-fetoprotein and periostin that were specific to newborn infants. Conclusion： These data will contribute to a better understanding of the composition of umbilical cord serum and aid the discovery of novel biomarkers for the prenatal diagnosis of fetal abnormalities.

关 键 词：mass spectrometry reference specimen serum proteome umbilical cord serum 

分 类 号：Q51[生物学—生物化学] Q581