ELISA与小白鼠生物法检测腹泻性贝类毒素的研究  

Diarrihetic shellfish poison detecting in scallop by ELISA and mouse biological method

作　　者：赵前程[1,2] 来鹏[2,3] 秦成[2,3] 王翀[4] 吴斌 刘迪[2] 常亚青[1] 

机构地区：[1]大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室,大连116023 [2]大连水产学院食品工程学院辽宁省水产品加工及综合利用重点开放实验室,大连116023 [3]辽宁省出入境检验检疫局,大连116001 [4]沈阳农业大学食品学院,沈阳110161

出　　处：《食品科技》2009年第11期287-292,共6页Food Science and Technology

基　　金：农业部海洋水产增养殖学与生物技术重点开放实验室开放课题(k2006-08)

摘　　要：以辽宁地区扇贝为原料,采用传统小白鼠生物实验法和酶联免疫吸附法对扇贝中的腹泻性贝类毒素残留量进行了检测。结果表明:扇贝样品中检测到腹泻性贝类毒素,小鼠法检测出样品中含有腹泻性贝类毒素为0.3MU/g,酶联免疫吸附法检测到样品中腹泻性贝类毒素含量为0.409μg/g。实验又将2种方法进行了比较,得出结论:2种方法结合起来作为测定腹泻性贝类毒素的方法,可以确保得到的结果更精确,更具说服力。In this paper, Scallop in Liaoning area was used as a raw material, and mouse biological method and ELISA method were adopted to detect diarrhetic shellfish poisoning（DSP） residue in scallop. The result indicated that the scallop sample had diarrhea shellfish toxin, and the DSP content inspected by mouse biological method and ELISA method were 0.3 MU/g and 0.409 μg/g, respectively. Comparing the two methods, the conclusion could be made that the combination of them to determine DSP would be more precise and persuasive in detecting DSP.

关 键 词：腹泻性贝毒 酶联免疫吸附法 小白鼠生物法 

分 类 号：R99[医药卫生—毒理学]