不同方法提取瑶药千斤拔挥发油的对比研究  

Comparative Research on Volatile Oil Extracted from Yao Medicine Flemingia Philippinensis Merr.et Rolfe.by Different Methods

作　　者：范贤[1] 王永良[1] 李玉兰[1] 黄秋燕[1] 伍秋明[2] 岑颖洲[1] 

机构地区：[1]暨南大学化学系,广东广州510632 [2]暨南大学分析测试中心,广东广州510632

出　　处：《精细化工》2009年第11期1085-1089,1144,共6页Fine Chemicals

基　　金：国家自然基金项目(20472024;20772047);广东省自然科学基金项目(039213);广东省中医药局立项课题(2008314)~~

摘　　要：分别采用水蒸气蒸馏(SD)法、超声萃取水蒸气蒸馏(UESD)法、超临界CO2流体萃取(SFE-CO2)法提取瑶药千斤拔的挥发油。对这3种方法所提取的挥发油的化学成分及其抑制人体肺癌细胞A549的活性进行分析比较。实验结果表明:SD法、UESD法和CO2-SEF法提取的挥发油的收率分别为0.03%,0.08%和0.5%。SD法和UESD法提取的主要化学成分为萜烯类的物质,而CO2-SEF法提取的主要化学成分是醇和酸类物质。在质量浓度为150 mg/L时,SD法提取的挥发油对肿瘤细胞的抑制率达到了69.13%,UESD法为59.91%,而CO2-SEF法提取的挥发油对肿瘤细胞A549的抑制率仅有24.08%。通过综合比较分析发现,挥发油中的雪松烯和长(松)叶烯-(V4)可能是抑制肺癌细胞A549的主要活性成分。其新颖性已为暨南大学图书馆2009年7月8日出具的第JNUL20090057C号《科技查新报告》所证实。Volatile oils were exacted by the steam distillation（SD） method,the ultrasound-assisted extraction and steam distillation（UESD） method and the CO2 supercritical fluid extraction（SFE-CO2） method from Yao medicine Flemingia philippinensis Merr.et Rolfe.The chemical composition and the antitumor activity on human lung cancer cells A549 were analyzed and compared.The oil yield obtained by SD was 0.03%,by UESD was 0.08% and by CO2-SEF was 0.5%.The main components of volatile oils extracted by SD and UESD were terpenoids,but the main components extracted by CO2-SEF were alcohols and acids.At the volatile oil mass concentration of 150 μg/mL,the inhibition rate of volatile oil exacted by SD on tumor cell A549 reached 69.13%,by UESD achieved 59.91%,but the inhibitory rate of volatile oil exacted by CO2-SEF only reached 24.08%.Through a comprehensive comparative analysis showed that the main active ingredients inhibiting A549 lung cancer cells may be Himachalene and longifolene-（V4） in the volatile oil and its novelty was confirmed in Technology Novelty Search Report No.JNUL20090057C by the Library of Jinan University on July 8,2009.

关 键 词：瑶药千斤拔 挥发油 水蒸气蒸馏法 超声萃取水蒸气蒸馏 超临界CO2流体萃取 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]