雏蝗属(Chorthippus)六种蝗虫基因组DNA的RAPD比较研究  

Comparative Studies of Genomic DNA of Six Grasshopper in Genus Chorthippus Using RAPD Markers

作　　者：谭竹钧[1,2,3,4] 韩雅莉[1,2,3,4] 苏和风 郑哲民 

机构地区：[1]内蒙古大学生物学系 [2]内蒙古农牧学院草原系 [3]呼和浩特市工商银行医务室 [4]陕西师范大学生物学系

出　　处：《内蒙古大学学报（自然科学版）》1998年第6期801-805,共5页Journal of Inner Mongolia University：Natural Science Edition

基　　金：内蒙古科委自然科学基金

摘　　要：运用随机扩增多态ＤＮＡ技术对雏蝗属６种蝗虫基因组ＤＮＡ进行了多态性比较研究，共扩增出１２条特异性片段，分子量大小约为７４０～１８１０ｂｐ，并根据各种间片段共享度构建了ＵＰＧ聚类关系图．根据研究结果推测，短翅亚属的小翅雏蝗和北方雏蝗分化较晚、相似性很高，它们可能起源于同一祖先；Polymorphism of genomic DNA in six grasshoppers of genus Chorthippus was a studied using random amplified polymorphic DNA (RAPD) in this paper.Twelve DNA segments were amplified with primer OPY-07,their molecular weight was 740 bp to 1 810 bp.Dendrogram of six species was constructed based on proportion of the fragments.Relationships among six species were examined by comparison the fragments.The results suggest that Chorthippus fallax (Zub.) and Chorthippus hammars stoemi (Mir.) may come from a common ancester and lately divide.Difference among four species of Glyptobothrus chop. was more obvious.

关 键 词：蝗虫 雏蝗属 RAPD 种系发生 DNA 

分 类 号：Q969.265.2[生物学—昆虫学]