三种促甲状腺素受体抗体检测试剂的比较  

Comparison of the Sensitivity of Serum TRAb Assay with Three Different Kits

作　　者：高云朝[1] 钱晓煜[1] 葛雯莉[1] 

机构地区：[1]上海交通大学第六人民医院核医学科,200233

出　　处：《放射免疫学杂志》2009年第4期405-407,共3页Journal of Radioimmanology

摘　　要：目的:评价某国产促甲状腺素受体抗体(TRAb)检测试剂的临床诊断性能,为临床应用提供指导。方法:应用国产TRAb试剂与两种进口同种试剂(德国Roche和英国RSR)同时检测77份临床样本,对比分析三种试剂检测结果的相关性、符合率,并结合历史资料回顾,计算国产试剂对Graves’病的诊断敏感性。结果:两种进口试剂所测TRAb在Graves’病的阳性比例均为13/15,对本文样本阴、阳性分类符合率为89.6%,总体相关系数为0.767(P<0.001)。国产TRAb试剂在本文Graves’病阳性比例为2/15,在历史回顾病例的阳性比例为5/31;与两种进口试剂比较符合率<60%、检测浓度相关系数<0.2(P>0.1)。结论:两种进口TRAb试剂对Graves’病具有较高的临床诊断性能并相互吻合,某国产TRAb试剂缺乏临床应用价值,建议摒弃。Objective To assess the sensitivity of TRAb assay with a new local made kit.Methods Seventy-seven serum specimens（including 15 specimens from confirmed Graves＇ disease patients） were tested for TRAb with three different kits（Roche,ESR and local made）.Results In the 15 specimens from patients with Graves＇ disease,TRAb was positive in 13 specimens with both Roche and ESR kits.However,with local made kit,TRAb was only positive in 2 specimens.The sensitivity of TRAb assay with local made kit was obviously much inferior to that with the other two kits.Another 31 specimens from patients with Graves＇s disease were tested with local made kit and only 5 were found TRAb positive.Conclusion The local made kit for TRAb assay（with different mechnism from that of the other two kits） seems to be much less sensitive and there fore not clinically useful.

关 键 词：甲状腺素受体抗体 Graves’病 临床诊断性能 

分 类 号：R581.1[医药卫生—内分泌] R512.630.4[医药卫生—内科学]