高效液相色谱-柱前衍生法测定水中有机磷除草剂  

Determination of Organophosphorus Herbicide in Water by high Performance Liquid Chromatography-pre-column Derivatization

作　　者：刘铮铮[1] 李立[2] 王静[1] 潘荷芳[1] 

机构地区：[1]浙江省环境监测中心,浙江杭州310012 [2]树人大学生物与环境学院,浙江杭州310015

出　　处：《中国环境监测》2009年第5期35-38,共4页Environmental Monitoring in China

基　　金：浙江省科技厅重大科技专项社会发展重点项目(2006C13060)

摘　　要：建立了水样中痕量草甘膦、草铵膦和氨甲基磷酸的FMOC柱前衍生-高效液相色谱-荧光检测分析方法。草甘膦,草铵膦和氨甲基磷酸的平均加标回收率分别为94.2%、90.8%、98.6%;相对标准偏差分别为4.8%、0.68%、2.8%;方法的定性下限和定量下限分别为0.05、0.04、0.009μg/L和0.16、0.12、0.03μg/L。水样放置两天后,水样中的草甘膦、草铵膦分别降解了17.8%和19.5%,而水样经过衍生后在5天内是稳定的。A method is proposed that allows the simultaneous determination of residue glyphosate, glufosinate and aminomethylphosphonic acid （AMPA） in water samples. The method involves pre-column derivatization step with 9-fluorenylmethylchloroformate （FMOG） in borate buffer and detection based on high performance liquid chromatography （HPLC） coupled with fluorophotomeric detectior. The method produces satisfactory reliability, sensitivity, and accuracy. The mean recovery values are 94.2 %, 90.8 %, 98.6 % for glyphosate, glufosinate and AMPA, respectively, and the relative standard deviation are 4.8% ,0.68% ,2.8%, respectively. Limits of detection and quantitative lower limits are 0.05, 0.04, 0.009μg/L and 0.16, 0.12, 0.03μg/L for the three compounds. The degradation rate of glyphosate, glufosinate in water are 17.8% and 19.5% after 2 days. The derivatization of these compounds are stable in 5days.

关 键 词：草甘膦 草铵膦 氨甲基磷酸 高效液相色谱 荧光 衍生 

分 类 号：X832[环境科学与工程—环境工程]