西归多糖含量测定方法的比较  

A Comparison of Methods for the Determination of Polysaccharides in Seselopsis

作　　者：周萍[1] 王成军[1] 林剑军[1] 秦菁莉[1] 卢峰[1] 

机构地区：[1]大理学院药学院,云南大理671000

出　　处：《大理学院学报（综合版）》2009年第10期10-12,共3页Journal of Dali University

基　　金：大理学院应用开发研究基金资助项目(KY421740)

摘　　要：目的:测定西归中多糖的含量。方法:用蒽酮-硫酸法及苯酚-硫酸法对西归中的多糖含量进行测定,并对两种方法的结果进行比较。结果:蒽酮-硫酸法平均加样回收率为86.6%,RSD=2.50%(n=6)。苯酚-硫酸法平均加样回收率为101.0%,RSD=2.63%(n=6)。结论:苯酚-硫酸法优于蒽酮硫酸法。Objective： To detect polysaccharides from Seselopsis. Methods： Phenol-sulfuric acid method and Anthrone-sulphuric acid method were adopted to survey the content of polysaccharide in Seselopsis, and the results of two methods were compared. Results：The average recovery rate of anthrone-sulphuric acid method was 88.6%,RSD=2.50%（n=6）; phenol-sulfuric acid method was 101.0%, RSD =2.63%（n=6）. Conclusion：Phenol-sulfuric acid method is superior to anthrone -sulphuric acid method.

关 键 词：西归 多糖 苯酚-硫酸法 蒽酮-硫酸法 

分 类 号：Q949.763.3[生物学—植物学]