藏獒血液蛋白多态性研究  

Study on the Protein Polymorphism in the Blood of Tibetan Mastiff

作　　者：兰小平[1] 郭宪[2] 陈永昌[3] 鄢珣[4] 崔泰保[4] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741000 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [3]中国科学院昆明动物研究所,云南昆明650223 [4]甘肃农业大学,甘肃兰州730070

出　　处：《安徽农业科学》2009年第33期16274-16276,共3页Journal of Anhui Agricultural Sciences

基　　金：甘肃省科技厅基金项目(GKC-97-27-5);天水师范学院青年基金资助项目(X4-25)

摘　　要：[目的]了解藏獒血液蛋白基因座的遗传变异状况,为藏獒品种资源保护及合理开发利用提供理论依据。[方法]采用不连续垂直板聚丙烯酰胺凝胶电泳技术,对河曲藏獒、青海藏獒、青海藏狮犬和青海土种犬共4个群体103只犬的7个血液蛋白基因座(Tf、Po、Sα2、Hb、Alb、Pr、Amy)的多态性进行研究,并分析不同群体的群体内遗传变异。[结果]4个犬群中,Tf、Po和Sα23个基因座上存在多态性,其中Tf和Po分别由3个等位基因控制,Sα2由2个等位基因控制,Hb、Alb、Pr和Amy基因座均呈现单态;藏獒群体的有效等位基因数(Ne)和Nei氏平均预期基因杂合度(H)分别为1.5324和0.2303,均高于其他犬群。[结论]藏獒群体内在血液蛋白位点上存在丰富的遗传变异。[ Objective] The aim was to find out the genetic variations in blood protein of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff. [ Method ] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein loeus（Tf, Po, Sα2, Hb, Alb, Pr and Amy）were investigated by using vertical polyaerylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei＇ s expected average heterozygosity were 1. 532 4 and 0. 230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Genetic variations within population in blood protein lociof Tibetan Mastiff were richer than other populations.

关 键 词：藏獒 血液蛋白多态性 有效等位基因数 Nei氏平均预期基因杂合度 

分 类 号：S829.2[农业科学—畜牧学]