反义抑制ghrelin对VEGF及其受体Flt-1 mRNA表达的影响  

Effects of Ghrelin Antisense Inhibition on VEGF and Its Receptor Flt-1 mRNA Expression

作　　者：姜丽萍[1,2,3] 祝啸先 游存厚 乌日古木拉 王芳 张文娟[3] 刘德斌 杜晨光[1] 李海军[1] 包福祥[1] 赵鹏伟[1] 鲍庆江[1] 曹贵方[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古医学院,内蒙古呼和浩特010059 [3]内蒙古自治区医院,内蒙古呼和浩特010017 [4]内蒙古妇幼保健院,内蒙古呼和浩特010020 [5]内蒙古自治区人民检察院技术处,内蒙古呼和浩特010011

出　　处：《安徽农业科学》2009年第33期16277-16279,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30860201)

摘　　要：[目的]探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Flt-1mRNA表达的影响。[方法]试验分为4组:I组,空白对照组;II组,脂质体组;III组(SCON),20μmol/L正义寡核苷酸组;IV组(ASCON),20μmol/L反义寡核苷酸组。上述各组均在培养24、36、48h后,采用实时荧光定量方法检测VEGF及其受体Flt-1mRNA表达情况的变化。[结果]VEGFmRNA在I、II组表达量无差异,具有较高的表达量,随着时间的延长无明显变化;在III组VEGFmRNA表达略有降低,但与I和II组间无显著性差异(P>0.05);IV组(反义寡核苷酸组)的VEGFmRNA表达量显著下降(P<0.05),并且随着时间延长逐渐下降。VEGF受体FLT-1mR-NA的表达与VEGF相似。[结论]反义抑制ghrelin对VEGF及其受体Flt-1的mRNA表达有下调作用。[ Objective ] This study was to investigate the effect of VEGF and its receptor Fh-1 mRNA expression in Mongolia sheep＇ s umbilical vein endothelial cells by ghrelin antisense inhibition. [ Method] Experiments were divided into 4 groups：group Ⅰ （blank control group） ; group Ⅱ （liposome group） ; group Ⅲ（SCON group： 20 μmol/L sense oligonucleotide） ; group Ⅳ （ASCON： 20 μmol/L antisense oligonucleotide）. VEGF and its receptor Fh-1 mRNA expression changes were detected by using real-time fluorescence quantitative detection after 24, 36 and 48 h. [ Result] The expression of VEGF mRNA in group Ⅰ, group Ⅱwere insignificantly different at higher expression levels, and did not change significantly with the time; the expression of VEGF mRNA in group Ⅲ assumed a slight decrease, but there were no significant differences between group Ⅰ and group Ⅱ （P 〉 0.05 ）, the expression of VEGF mRNA in group Ⅳ （ antisense oligonucleotide group ） decreased significantly （ P 〈 0.05 ） ; the expression of VEGF receptor FLT-1 mRNA was similar to that of VEGF [ Conclusion ] Antisense inhibition ghrelin has a downward effect to the expression of VEGF and its receptor Fh-1.

关 键 词：生长素 血管内皮生长因子 受体 寡核苷酸类 反义 

分 类 号：S188[农业科学—农业基础科学]