灵芝多糖对糖尿病大鼠肾组织TGF-β_1和CO-Ⅳ表达的影响  

Effects of Ganoderma lucidum Polysaccharides on the Expression of Transforming Growth Factor-Beta1、Type Ⅳ Collagen in Kidney Tissue of Diabetic Rat

作　　者：毛春谱[1] 李小毅[1] 张红梅[1] 林桂芬[1] 李伟[2] 

机构地区：[1]苏州大学附属无锡第四医院,江苏无锡214062 [2]江苏省徐州医学院附属医院,江苏徐州221006

出　　处：《时珍国医国药》2009年第11期2678-2680,共3页Lishizhen Medicine and Materia Medica Research

基　　金：江苏省中医药局资助项目(No.H05102)

摘　　要：目的观察转化生长因子β1(TGF-β1)及Ⅳ型胶原(CO-Ⅳ)在糖尿病大鼠肾组织中的表达及灵芝多糖干预后的影响。方法腹腔注射链脲佐菌素(STZ)诱导大鼠造成糖尿病模型,分组予不同剂量灵芝多糖(GLP)进行治疗性灌胃。8周后,用免疫组化方法和RT-PCR方法检测各组大鼠肾皮质TGF-β1、CO-Ⅳ的蛋白和mRNA表达。结果糖尿病组大鼠肾小球TGF-β1、CO-Ⅳ蛋白和mRNA表达较正常对照组明显升高(P<0.01);灵芝多糖各组较糖尿病组表达降低(P<0.01)。结论灵芝多糖可能通过下调糖尿病大鼠肾脏中TGF-β1和CO-Ⅳ的表达,减少细胞外基质积聚,起到对糖尿病大鼠肾脏保护作用。Objective To investigate the expressions of transforming growth factor-Beta1（TGF-β1） and type Ⅳ collagen（CO-Ⅳ） in the kidney of diabetic rats and the effects of Ganoderma lucidum polysaccharides（GLP）on such changes.Methods Diabetic rat model was induced by streptozocin（60mg/kg）and the rats were divided into 5 groups randomly and treated with different dose GLP.8 weeks later,the expression of TGF-β1 and CO-Ⅳ in kidney tissue was determined by immunohistochemistry and RT-PCR methods.Results Compared with group C ,the expressions of protein and mRNA TGF -β1 ,CO -Ⅳ significantly in creased in glomeruli （ P 〈 0.01 ）. The abnormal expressions of these proteins, mRNA were dramatically improved in diabetic rats by GLP treatment（ P 〈 0.01 ）. Conclusion GLP has some renalprotective effect on diabetic rats ,through inhibition of excessive deposition of glomeruli extracellular matrix by inhibiting the expression of TGF-β1 and decreasing the synthesis of CO - Ⅳ.

关 键 词：灵芝多糖 糖尿病肾病 转化生长因子Β1 Ⅳ型胶原 

分 类 号：R284.2[医药卫生—中药学]