DNA分子标记在中药鉴定中的应用及发展趋势分析  

Application of DNA Molecular Markers in Chinese Medicinal Materia Identification and its Development Trends

作　　者：李力[1] 徐永莉[1] 张月云[1] 赵成坚[1] 

机构地区：[1]中国医学科学院药用植物研究所广西分所,广西南宁530023

出　　处：《时珍国医国药》2009年第11期2845-2847,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技支撑计划(No.2006BAI06A11-01);广西科学研究与技术开发计划项目(No.0815005-2-16)

摘　　要：目的对DNA分子遗传标记技术在中药材鉴定领域的应用作一综述。方法通过查阅20世纪90年代以来发表的文献进行归纳分析。结果DNA分子遗传标记技术具有微量、快速、特异性强、准确可靠、对样本要求较低的特点;使用较多的方法主要有RAPD、ISSR、ITS标记以及rRNA基因序列分析技术。同时,DNA分子标记技术也存在着使用局限、对实验硬件和从业人员的技术要求高、成本较高、技术复杂度高、对不同部位入药的药材仍然难以区分以及无法用于成药、复方、提取液鉴定等弱点。结论DNA分子遗传标记作为中药材鉴定的方法还有待发展和完善。Objective The paper summarizes the researches of application of DNA molecular markers in Chinese medicinal materia.Methods Induction and analysis were used by referring to literature released since 1990s.Results DNA Marker Technologies have characteristics of trace,rapid,peculiarity,accuracy and credibility,lower requirements for the sample.Random amplified polymorphic DNA（RAPD）,inter-simple sequence repeat（ISSR）,interial transcribed spaoers（ITS） and rRNA gene sequencing technology are often used. However, DNA Marker Technologies have some disadvantages that employees are required special skills, the cost is high and the technique is complicated ,while it is difficult to distinguish different parts of Chinese medicinal materia used medicinally and identify patent medicine, Chinese medicine complex prescription and the extract of Chinese medicinal materia. Conclusion The methods of Chinese medicinal materia identification by DNA molecular markers need to develop and improve.

关 键 词：DNA分子遗传标记 中药材 鉴定 

分 类 号：R282.5[医药卫生—中药学]