麝香乌龙丸对兔骨关节炎软骨细胞凋亡的影响  

The Effect of Shexiang-wulong Pill on the Rabbit Osteoarthritis Chondrocyte Apoptosis

作　　者：王志文[1] 赵鑫[2] 鲍际鹏[2] 谷宁飞[2] 王建纲[2] 

机构地区：[1]华北煤炭医学院附属医院,河北唐山063000 [2]华北煤炭医学院,河北唐山063000

出　　处：《时珍国医国药》2009年第11期2893-2894,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的观察麝香乌龙丸对兔膝骨关节炎软骨细胞凋亡和凋亡调控基因bcl-2及其抑制基因bax的影响,探讨麝香乌龙丸对关节软骨的保护作用是否与抑制细胞凋亡与调节bcl-2及bax的表达有关。方法选用健康日本大耳白兔30只,随机分成正常对照组、造模组、麝香乌龙丸组。用1.6%木瓜蛋白酶膝关节腔内注射,建立兔膝骨关节炎模型,4周后处死全部动物,取右膝关节胫骨内侧髁软骨,软骨组织切片用TUNEL法检测软骨细胞凋亡,免疫组化法检测关节软骨中bcl-2、bax的水平。结果麝香乌龙丸组细胞凋亡率明显低于造模组(P<0.05),bcl-2在麝香乌龙丸组中的表达高于造模组(P<0.05),bax的表达低于造模组(P<0.05)。结论麝香乌龙丸通过上调bcl-2的表达,下调bax的表达,达到抑制兔实验性膝骨关节炎软骨中软骨细胞的过度凋亡,这可能是该药治疗骨性关节炎机制之一。Objective To observe the effect of Shexiang-wulong pill on the rabbit knee osteoarthritis（KOA） chondrocyte apoptosis,apoptosis regulatory genes bcl-2 and its impact suppressor gene bax.To explore the relationship of Shexiang-wulong pill on the protective effect of articular cartilage with the inhibition of apoptosis and regulation bcl-2 and bax expression.Methods 30 healthy male rabbits were randomly divided into three groups： A.normal control group,B.model group,C.Shexiang-wulong pill group. Make KOA models by injecting papain to the right knees of rabbits, after 4 weeks all animals were put to death. Then take the right knee medial tibial condyle cartilage. Cartilage tissue with cartilage TUNEL assay of apoptosis, detected by immunohistochemistry of articular cartilage in the bcl - 2, bax level. Results The rate of apoptosis of the group C was significantly lower than of B （ P 〈 0.01 ）, the expression of bcl - 2 in C was higher than that in B （ P 〈 0.01 ）, the expression of bax in, the C was lower than in B （P 〈 O. 01 ）. Conclusion It plays a role by upregulating the expression of bcl - 2, reducing the expression of bax to inhibit the excessive apoptosis of chondrocytes on the rabbit KOA, this may be one mechanism of Shexiang - wulong pill on the treatment of KOA.

关 键 词：麝香乌龙丸 膝骨关节炎 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]