新兵260名血清中艾滋病抗体检测假阳性结果分析  

Analysis of False Positive Results of HIV Serum Antibody in 260 Cases from Recruits

作　　者：俞苏蒙[1] 高彦军[1] 叶晓波[1] 冯莉[1] 高娅玲[2] 

机构地区：[1]中国人民解放军北京军区空军后勤部防疫队,100005 [2]北京军区空军后勤部卫生处

出　　处：《职业与健康》2009年第23期2575-2577,共3页Occupation and Health

摘　　要：目的了解新兵艾滋病抗体初筛检测中假阳性结果真实情况,探讨初筛实验与确认实验之间关系,为提高艾滋病检测技术提供依据。方法采用酶联免疫吸附试验(ELISA)技术,对2000—2007年期间应征入伍的新兵进行艾滋病抗体初筛检测,阴性者判定阴性,阳性者再用另外一种试剂复核,复核阳性标本再送全军艾滋病检测中心采用免疫印迹试剂进行确认。结果6年间共检测新兵血清标本49017份,筛查出260份阳性血清标本,发现初筛所能够捡出的全部抗-HIV阳性标本,经复核和确认实验后,100%为HIV抗体阴性,(S/CO)值在1.000～5.000范围的249份,占95.8%;超过5以上的11份,占4.2%。结论新兵人群艾滋病病毒抗体检测存在一些假阳性结果,复核实验是有效排除假阳性、提高确认效率、节省经费开支的有效措施。[ Objective ] To understand the false positive results of HIV antibody screening in recruits, to explore the correlation between the initial screening and the confirmation test, and provide evidence for improving HIV detection techniques. [ Methods ] ELISA method was adopted to conduct initial screening of HIV antibody in recruits during 2000--2007, negative samples were judged as negative, all positive samples were re - examination by another reagent, the confirmed positive cases were sent to the mili- tary HIV control center for further confirmation by western blot. [ Results ] Totally 49 017 serum samples of recruits were examined for HIV antibody during 6 years. The initial screening showed 260 positive results which were 100% negative after re-examination and confirmation experiments. The S/CO values （ 249 false positive samples ） were between 1. 000 - 5. 000, accounting for 95.8%, 11 samples＇S/CO values were more than 5, accounting for 4.2%. [ Conclusion] There are some false positive results for HIV antibody in the recruits. Re-examination is an efficient practice to eliminate the false positive results from the initial screening, to improve the efficiency of HIV detection, and to save budget.

关 键 词：艾滋病 血清抗体 新兵 

分 类 号：R446.62[医药卫生—诊断学]