中药单体齐墩果酸、姜黄素和大蒜素增强宫颈癌Me180细胞放疗敏感性  

Effect of radiation combined with Chinese medicinal monomers on Me180 cells

作　　者：耿传营[1] 徐波[1] 李红艳[1] 陈志华[1] 夏启胜[1] 徐梅[1] 刘轩[1] 刘玉风[2] 向青[1] 

机构地区：[1]中日友好临床医学研究所生化室,北京100029 [2]北京市大兴区人民医院口腔科

出　　处：《中华放射医学与防护杂志》2009年第5期472-475,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究放射联合中药单体对人宫颈癌（Me180）细胞增殖、端粒酶活性、细胞周期相关基因表达的作用。方法利用齐墩果酸、姜黄素和大蒜素3种中药单体作用于Me180细胞，MTr法检测中药单体对Me180细胞的杀伤作用，端粒重复序列扩增法（TRAP）法检测中药单体对Me180细胞端粒酶活性的抑制作用，实时PCR法研究中药单体对Me180细胞周期相关基因的表达情况，总量6Cy剂量率为300MU／min的X线联合中药单体治疗，观察对Me180细胞的杀伤作用。结果射线联合中药单体作用于Me180细胞后，3种中药单体使细胞增殖率明显降低（t=2．81、4．16、3．42，P〈0．05）。中药单体对肿瘤Me180细胞端粒酶活性有抑制作用，且有一定的时相性，16h后影响最为明显，齐墩果酸和大蒜素使端粒酶活性明显减低（t=5．11、5．29，P〈0．05）；48h后端粒酶活性基本恢复正常水平。齐墩果酸作用后，Me180细胞p21和p16基因的表达分别为对照组的2．43倍和2，78倍，eyclin D1和CDK4基因的表达为对照组的56％和41％。结论射线联合中药单体可有效杀伤Me180细胞，抑制Me180细胞端粒酶活性，调节细胞周期相关基因的表达。Objective To observe the effect of radiation treatment combined with Chinese medicinal monomers on the proliferation function, telomerase activity, expressions of apoptosis- and proliferation-related genes of Me180 cells. Methods Me180 cells were cultured in the medium with oleanolic acid, eureumin and allicin. The survival rates of ceils were detected by the methods of MTT, the telomerase activity by the method of telomeric repeat amplification protocol （TRAP） and the apoptosis -and proliferation-related genes by the method of reverse transcriptase-PCR. Me180 cells were cultured in the medium with Chinese medicinal monomers, and exposed to X-ray irradiation and the survival rates were detected. Results The results of MTT showed that survival rates of tumor cells exposed to X rays in combination with aleanolic acid, curcumin and allicin were decreased significantly （ t = 2.81, 4.16, and 3.42, P 〈 0.05）. Chinese medicinal monomers inhibited the telomerase activity of Me180 cells and the inhibiting function changed with time. At 16 h, the telomerase activities of Me180 cells administered with oleanolic acid and allicin were reduced markedly （ t = 5.11 and 5.29, P 〈 0.05）. After 48 h, the telomerase activities returned to the normal level. The gene expressions of p21 and p16 in Me180 cells treated with oleanolic acid were 2.43 and 2.78 times higher than the control, respectively, while those of cyclin D1 and CDK4 were 56% and 41% of the control, respectively. Conclusions Chinese medicinal monomers could effectively kill tumor cells, inhibit the telomerase activity and the expression of proliferation-related genes, and enhance the radiosensitivity of tumor cells.

关 键 词：放射增敏 端粒酶 中药单体 

分 类 号：R285[医药卫生—中药学]