中药墨旱莲扶正固本和保肝作用的研究  

Studies on strengthening and restoring effects and hepatoprotective effect of Eclipta

作　　者：徐汝明[1] 邓克敏[1] 陆阳[1] 

机构地区：[1]上海交通大学医学院药学系,上海200025

出　　处：《上海交通大学学报（医学版）》2009年第10期1200-1204,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的评价中药墨旱莲的扶正固本和保肝作用。方法60只昆明小鼠随机分为对照组,墨旱莲高、低剂量组,环磷酰胺模型组,环磷酰胺(CY)模型的墨旱莲高、低剂量组,每组10只。以8 g.kg-1.d-1和4 g.kg-1.d-1剂量的墨旱莲灌胃给药7 d,给药第4天腹腔注射0.05 g/kg剂量的CY建立免疫低下模型,观察墨旱莲提取物对正常小鼠和CY诱导的免疫低下小鼠的胸腺指数、脾脏指数、碳粒廓清指数的影响;取BALB/c小鼠2只观察墨旱莲提取物对淋巴细胞增殖作用和ConA诱导小鼠淋巴细胞转化增殖作用的影响,评价墨旱莲扶正固本作用。70只昆明小鼠随机分为对照组,墨旱莲高、低剂量组,CCl4模型组,联苯双酯(DDB)阳性对照组,CCl4模型的墨旱莲高、低剂量组,每组10只小鼠。以20 g.kg-1.d-1和10 g.kg-1.d-1剂量的墨旱莲灌胃给药7 d,末次给药后2 h腹腔注射10 mL/kg剂量的CCl4建立肝损伤模型,观察墨旱莲提取物对正常小鼠和CCl4诱导的肝损伤小鼠血清ALT、MDA、TP、T-Bil值的影响;取SD大鼠1只,观察墨旱莲提取物对大鼠原代肝细胞活力的影响,评价墨旱莲的保肝作用。结果墨旱莲提取物能上调正常小鼠和CY诱导的免疫低下小鼠的胸腺指数和碳粒廓清指数;能促进小鼠淋巴细胞增殖以及ConA诱导的T淋巴细胞转化增殖;能降低肝损伤小鼠的血清ALT、MDA值;能增强原代培养肝细胞的活力。结论中药墨旱莲具有扶正固本作用和保肝作用。Objective To evaluate the strengthening and restoring effects and hepatoprotective effect of Eclipta extracts.Methods Sixty Kunming mice were randomly divided into control group,low-dose Eclipta group,high-dose Eclipta group,cyclophosphamide（CY） group,low-dose Eclipta CY group and high-dose Eclipta CY group（n=10）.Eclipta 8 g·kg^-1·d^-1 or 4 g·kg^-1·d^-1 were administered by oral gavage for 7 d.Immunosuppression mouse models were established by intraperitoneal injection of 0.05 g/kg CY on the fourth day of administration.The effects of Eclipta extracts on thymus index,spleen index and carbon clearance index in both normal mice and immunosuppression mice induced by CY were tested,and those on proliferation of lymphocyte and T cells induced by ConA were examined.Based on these data,strengthening and restoring effects of Eclipta were observed.Besides,70 Kunming mice were randomly divided into control group,low-dose Eclipta group,high-dose Eclipta group,CCl4 group,DDB group,low-dose Eclipta CCl4 group and high-dose Eclipta CCl4 group.20 g·kg^-1·d^-1 and 10 g·kg^-1·d^-1 Eclipta were administered by oral gavage for 7 d.Hepatic injury mouse models were established by intraperitoneal injection of 10 mL/kg CCl4 2 h after the last administration.The effects of Eclipta extracts on serum ALT,MDA,TP and T-Bil levels in both normal mice and hepatic injury mice induced by CCl4 was tested,and those on the viability of the primary culture hepatocytes were determined.Based on these data,hepatoprotective effect of Eclipta was observed. Results Eclipta extracts increased thymus index and carbon clearance index in both normal mice and immunosuppression mice induced by CY,promoted proliferation of lymphocyte and proliferation of T cells induced by ConA,reduced serum ALT and MDA levels in hepatic injury mice,and improved the viability of the primary culture hepatocytes. Conclusion Eclipta herb has strengthening and restoring effects and hepatoprotective effect.

关 键 词：墨旱莲 环磷酰胺 CCL4 扶正固本 保肝 

分 类 号：R285.5[医药卫生—中药学] R967[医药卫生—中医学]