日本血吸虫和斯氏并殖吸虫的rDNA-ITS2遗传多态性研究  

Genetic polymorphism of rDNA-ITS2 of Schistosoma japonicum and Paragonimus skrjabini

作　　者：何谐[1] 张锡林[1] 牛靖萱[1] 陈琳[1] 

机构地区：[1]第三军医大学基础医学部病原生物学教研室,重庆400038

出　　处：《第三军医大学学报》2009年第21期2073-2077,共5页Journal of Third Military Medical University

基　　金：重庆市重大科技专项(CSTC2007AB5029);重庆市自然科学基金(CSTC2007BB5055)~~

摘　　要：目的分析日本血吸虫和斯氏并殖吸虫的核糖体DNA第二内转录间隔区(rDNA-ITS2)基因的遗传变异。方法采集2种吸虫成虫标本,提取其基因组DNA,PCR特异性扩增rDNA-ITS2基因并测序;Clusterx软件进行多序列对比;MEGA4软件计算遗传距离,NJ法和MP法构建系统进化树;DNAsis软件对rDNA-ITS2序列片段进行模序分析。结果2种方法构建的遗传系统进化树基本结构相似,日本血吸虫与斯氏并殖吸虫间存在较远的遗传距离,但二吸虫ITS2序列间有42.7%的相似性。利用DNAsis软件在日本血吸虫和斯氏并殖吸虫的rDNA-ITS2序列中发现AP_2_CS6、bHLH_CS和CAP_site3种相同的转录因子。结论日本血吸虫和斯氏并殖吸虫虽然在rDNA-ITS2基因水平上存在明显种间差异,但在rDNA-ITS2序列、转录因子方面具有重要的相似性。Objective To analyze the genetic variation of rDNA-ITS2 of Schistosoma japonicum and Paragonimus skrjabini in order to avoid the cross reactions in immune diagnosis between schistosomiasis and paragon-imiasis. Methods The 2 species of fluke were collected,and then the genomic DNA was extracted,to amplify the gene of rDNA-ITS2 by polymerase chain reaction （ PCR） and then sequenced. Multiple sequences were aligned by Clusterx,the genetic distance was analyzed by MEGA4,and the phylogentic tree of rDNA-ITS2 genes of Schistosoma and Paragonimus constructed with MP and NJ method. The motifis of the rDNA-ITS2 gene sequences of Schistosoma and Paragonimus were analyzed with DNAsis. Results The results of phylogentic trees of rDNA-ITS2 genes of Schistosoma and Paragonimus were similar. The genetic distance between Schistosoma japonicum and Paragonimus skrjabini was longer,though there was 42. 7% similarity between their rDNAITS2 gene. There were 3 same transcription factors in the sequences of Schistosoma japonicum as well as Paragonimus skrjabini,AP_2_CS6,bHLH_CS and CAP_site. Conclusion Though there are obviously differences between the Schistosoma japonicum and Paragonimus skrjabini at the level of gene,they have significantly similarity in transcription factors of rDNA-ITS2 sequences. It may provide valuable theoretical information for studies on cross-reacting antigen between Schistosoma japonicum and Paragonimus skrjabini at gene level.

关 键 词：日本血吸虫 斯氏并殖吸虫 RDNA-ITS2 序列 遗传多态性 

分 类 号：R383.24[医药卫生—医学寄生虫学] R394-33[医药卫生—基础医学]