大鼠腹腔海水浸泡伤后胃黏膜、血浆中一氧化氮和前列腺素的变化  

Dynamic changes of nitric oxide and prostaglandine levels in gastric mucosa and plasma in rats with seawater immersion after open abdominal trauma

作　　者：宋东华[1] 朱雄伟[2] 李兆申[3] 

机构地区：[1]解放军第411医院普通外科,上海200081 [2]武警总医院肝移植科,北京100039 [3]第二军医大学附属长海医院消化内科,上海200433

出　　处：《中国现代普通外科进展》2009年第9期749-751,共3页Chinese Journal of Current Advances in General Surgery

基　　金：全军医学科学技术研究"十五"计划重点课题基金资助项目(01Z059)

摘　　要：目的:探讨大鼠腹部开放伤海水浸泡后短时间内胃黏膜和血浆中一氧化氮和前列腺素的动态变化。方法:32只SD大鼠随机分成正常对照组和腹腔浸泡伤1、2、3h4组,取相应大鼠的胃黏膜和血浆,采用镉粒还原和比色法测定其中一氧化氮含量,采用放射免疫法测定其中前列腺素含量。结果:与正常组大鼠相比,腹腔浸泡伤大鼠胃黏膜和血浆中一氧化氮和前列腺素随着浸泡时间的延长而进行性升高(P<0.05),并在3h时达最高。结论:海水浸泡伤可引起大鼠胃黏膜和血浆中一氧化氮和前列腺素水平升高,提示一氧化氮和前列腺素在海水浸泡伤引起的急性胃黏膜病变发生发展过程中可能起重要作用。Objective： To explore the dynamic changes of nitric oxide and prostaglandine levels in gastric mucosa and plasma of rats with seawater immersion injury after open abdominal trauma Methods- Thirty-two Sprage-Dawley rats were randomly assigned into four groups： normal control group, celiac seawater-immersing trauma stress 1, 2, 3h groups The contents of nitric oxide and prostaglandine in gastric mucosa and plasma of rats were detected by chemochromometric and im- munoassay methods respectively Results： Compared with normal control group, both the nitric oxide and prostaglandine levels in gastric mucosa and plasma of rats under celiac seawater-immersing trauma were progressively increased （P〈0 05）, especially in stress 3h group Conclusion： Seawater immersing trauma is a harmful factor, it may cause the increasing levels of nitric oxide and prostaglandine in plasma and gastric mucosa, which may lead to acute gastric mucosal lesion in rats with open abdominal trauma after seawater immersion.

关 键 词：腹部损伤 海水 胃黏膜 一氧化氮 前列腺素 大鼠 Sprage-Dawley 

分 类 号：R656.6[医药卫生—外科学]