脂联素对L6大鼠骨骼肌细胞葡萄糖摄取的影响  

Effects of adiponectin on glucose uptake in L6 rat skeletal muscle cells

作　　者：刘元涛[1] 倪一虹[1] 姜兆顺[2] 陈诗鸿[1] 庄向华[1] 孙福敦[1] 李晓博[1] 潘喆[1] 宋慧玲[1] 

机构地区：[1]山东大学第二医院内分泌科,济南250033 [2]济南军区总医院内分泌科,济南250031

出　　处：《山东大学学报（医学版）》2009年第10期5-8,18,共5页Journal of Shandong University:Health Sciences

摘　　要：目的观察脂联素对L6大鼠骨骼肌细胞基础及胰岛素诱导的葡萄糖摄取的影响。方法1.将L6细胞分为脂联素处理组(脂联素刺激30min)、胰岛素处理组(10nmol/L胰岛素刺激20min)和对照组。处理后分别测定各组细胞的葡萄糖摄取率。2.将稳定表达葡萄糖转运子4(Gluosetransporter4,GLUT4)的L6细胞(L6-GLUT4细胞)分为6组:①对照组;②脂联素处理组:脂联素刺激30min;③低浓度胰岛素处理组:0.1nmol/L胰岛素刺激20min;④高浓度胰岛素组:10nmol/L胰岛素刺激20min;⑤脂联素预处理+低浓度胰岛素组:脂联素预处理30min,0.1nmol/L胰岛素刺激20min;⑥脂联素预处理+高浓度胰岛素组:脂联素预处理30min,10nmol/L胰岛素处理20min。处理后分别测定各组细胞的葡萄糖摄取率及GLUT4细胞膜含量。结果L6细胞:脂联素处理组葡萄糖摄取率与对照组差异无统计学意义(P>0.05)。L6-GLUT4细胞:①脂联素处理组细胞膜GLUT4含量、葡萄糖摄取率与对照组差异均无统计学意义(P均>0.05);②低浓度胰岛素处理组细胞膜GLUT4含量、葡萄糖摄取率均显著高于对照组(P均<0.01);脂联素预处理+低浓度胰岛素组细胞膜GLUT4含量、葡萄糖摄取率均显著高于脂联素处理组(P均<0.01)以及低浓度胰岛素处理组(P均<0.01);③高浓度胰岛素处理组葡萄糖摄取率、细胞膜GLUT4含量均显著高于低浓度胰岛素处理组(P<0.05,P<0.01);脂联素预处理+高浓度胰岛素组与脂联素预处理+低浓度胰岛素组比较,葡萄糖摄取率显著升高(P<0.05),而GLUT4细胞膜含量升高不显著(P>0.05);脂联素预处理+高浓度胰岛素组葡萄糖摄取率显著高于单纯高浓度胰岛素组(P<0.01),而GLUT4细胞膜含量差异无统计学意义(P>0.05)。结论①脂联素对骨骼肌细胞的葡萄糖基础摄取无明显影响;②脂联素本身不足以诱导骨骼肌细胞的GLUT4细胞膜转位及葡萄糖摄取;③脂联素可增加胰岛素诱导的骨骼肌细胞的葡萄糖摄取,Objective To observe the effects of adiponectin on basal and insulin-induced glucose uptake in L6 rat skeletal muscle myoblasts.Methods Wild-type L6 cells were divided into 3 groups：the adiponectin treatment group（stimulation with adiponectin for 30 min）,the insulin treatment group（stimulation with 10 nmol/L insulin for 20 min）and the control group.After treatments,the glucose uptake of each group was determined.L6 cells stably expressing GLUT4（Glucose transporter 4）were divided into 6 groups：the control group,the adiponectin treatment group（stimulation with adiponectin for 30 min）,the lower concentration of insulin group（stimulation with 0.1 nmol/L insulin for 20 min）,the higher concentration of insulin group（stimulation with 10 nmol/Linsulin for 20 min）,the adiponectin＋lower concentration of insulin group（pre-treatment with adiponectin for 30 min,then stimulation with 0.1 nmol/L insulin for 20 min）,and the adiponectin＋higher concentration of insulin group（pre-treatment with adiponectin for 30 min,then stimulation with 10 nmol/L insulin for 20 min）.After treatments,glucose uptake and plasma membrane GLUT4 content were determined.Results In wild-type L6 cells：the glucose uptake of the adiponectin treatment group was not significantly different from that of the control（P〉0.05）.In L6-GLUT4 cells：① Without insulin stimulation,the glucose uptake and the plasma membrane GLUT4 content of the adiponectin treatment group were not significantly different from those of the control（P〉0.05,respectively）.② The glucose uptake and the plasma membrane GLUT4 content of the lower concentration of insulin treatment group were both significantly higher than those of the control（P〈0.01,respectively）.With lower concentration of insulin treatment,the glucose uptake and the plasma membrane GLUT4 content of cells pre-treated with adiponectin were both higher than those of the adiponectin treatment group（P〈0.01,respectively）,and were also significantly higher than tho

关 键 词：脂联素 葡萄糖转运子4 骨骼肌细胞 葡萄糖摄取 

分 类 号：R587.1[医药卫生—内分泌] R774.1[医药卫生—内科学]