小鼠超排卵胚胎的着床与白血病抑制因子表达水平的关系  

The Relationship between the Implantation of Ovarian Stimulated Embryos and the Expression of Leukemia Inhibitory Factor Protein in Mice Metrium

作　　者：李培旭[1] 李尚为[2] 彭芝兰[2] 陈诵芬[1] 

机构地区：[1]济南军区总医院生殖中心,山东济南250031 [2]四川大学华西第二医院妇产科,四川成都610041

出　　处：《华西医学》2009年第9期2370-2372,共3页West China Medical Journal

摘　　要：目的:观察受体鼠妊娠和胚胎着床情况,并检测胚胎移植时小鼠子宫内膜中白血病抑制因子(Lif)表达水平,探讨超排卵对小鼠胚胎着床潜能的影响。方法:建立超排周期胚胎和自然周期胚胎移植小鼠模型,比较妊娠率、胚胎着床率的差异及其与Lif蛋白的表达水平之间的关系。结果:超排卵周期受体组的妊娠率(20.00%)和胚胎着床率(8.33%)显著低于自然周期组的妊娠率(55.00%)和胚胎着床率(35.00%)(P<0.05)。自然周期胚胎和超排周期胚胎受体组内膜中Lif蛋白的表达水平相似(P>0.05),妊娠受体组Lif蛋白的表达水平显著高于未孕受体组(P<0.05),但单胎妊娠和多胎妊娠受体组内膜中Lif蛋白的表达水平相似(P>0.05)。结论:超排卵可能降低胚胎的着床潜能,Lif蛋白的表达水平与胚胎着床有关,但与着床胚胎的数目无比例关系。Objective： To check the level of leukemia inhibitory factor protein （Lif-protein）, and to explore the impact of ovarian stimulation on the ability of embryo implantation in mice. Methods： Pregnancy models of mice were established, and the level of Lif-protein was determined by SP staining method. The difference of the pregnancy and im- plantation rate was compared between the ovarian stimulation and normal pregnancy models, and the relationship between Lif-protein levels and the pregnancy and implantation rate was analyzed. Results： Compared with the group of recipients which the transferred embryos were from non-stimulated cycles （55.00% and 35.00% respectively）, the group of recipients which the transferred embryos were from stimulated cycles had lower pregnancy and implantation rate （20.00% and 8.33% respectively） （P〈0.05）. The Lif-protein level had no significant difference between two groups （P〉0.05） and between single pregnancy group and multi-pregnancy group （P〉0.05）, but was higher in the group of pregnancy recipients than in the group of non-pregnancy recipients （P〈0.05）. Conclusion： Ovarian stimulation may reduce the the ability of embryo implantation in mice. Lif-protein level is related to implantation, but is not parallel to the number of implantation.

关 键 词：超排卵 妊娠率 胚胎着床率 Lif蛋白 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]