粉尘螨对过敏性支气管哮喘患者气道CD4^CD25^＋T细胞的募集作用  

Infiltration of CD4^＋ CD25^＋ T cells into the allergic asthmatic airways caused by house dust mite antigen administration

作　　者：陈一强[1] 黄红东[2] 温红侠[1] 邹小英[1] 邓静敏[1] 施焕中[1] 

机构地区：[1]广西医科大学呼吸病研究所,南宁530021 [2]湖南省人民医院

出　　处：《中华内科杂志》2009年第11期944-946,共3页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金（30460051）;广西科学基金项目（桂科回0342019）

摘　　要：目的研究过敏原粉尘螨提取液能否募集CD4^＋CD25^＋T细胞浸润到过敏性支气管哮喘（以下简称哮喘）患者气道。方法经纤维支气管镜将粉尘螨直接注入10例非急性发作期的轻度过敏性哮喘患者的右肺中叶或左肺舌叶的段支气管（粉尘螨侧），将生理盐水注入对侧肺段支气管作对照（对照侧）。24h后再经纤维支气管镜于相应的肺段支气管进行支气管肺泡灌洗，收集支气管肺泡灌洗液（BALF），计数BALF中的细胞数并分类。以流式细胞仪测定BALF中的T细胞及其亚群。结果粉尘螨侧BALF中嗜酸性粒细胞[（1．4±0．1）×10^6／ml]明显多于对照侧[（0．34±0．1）×10^6／ml，P〈0．003]，淋巴细胞[（2．2±0．3）×10^6／ml]明显多于对照侧[（0．3±0．1）×10^6／ml，P〈0．05]，CD4^＋CD25^＋T细胞[（784．0±281．3）个／μl]明显多于对照侧[（7．7±3．6）个／μl，P〈0．001]。结论粉尘螨能募集CD4^＋CD25^＋T细胞浸润到缓解期轻度过敏性哮喘患者气道局部。Objective To investigate whether house dust mite（HDM） could induce CD4^＋ CD25^＋ T cells infiltration into asthmatic airways in patients vivo. Methods Ten subjects with asthma underwent initial bronchoscopy during which normal saline and HDM were administered to two sublobar segments separately. The second bronehoscopy were carried out and bronchoal lavage fluid from HDM-challenged sites and saline-challenged sites were separately taken 24 h later. The differential cell counts were determined, and the absolute number of each type was calculated. At the same time, CD3^＋ ,CD4^＋ and CD4^＋ CD25^＋ T cells were determined by flow-cytometric analysis. We compared cellular counts in airways without and after topical instillation of HDM. Results Eosinophile granulocyte cells of broncho-alveolar fluid in the HDM- challenged sites（ 1.4 ±0. 1 ） ×10^6/ml are more than it in control sites（0. 3 ±0. 1 ） ×10^6/ml, P 〈0. 003. Lymphocyte cells of BALF in the HDM-challenged sites （2. 2 ±0. 3 ） ×10^6/ml are more than it in control sites（0. 3±0. 1 ） ×10^6/ml, P 〈 0. 001; CD4^＋ CD25^＋ T cells of BALF in the HDM-challenged sites （784. 0 ± 281.3 ）cell/μl are more than it in control sites（7.7 ± 3.6）cell/μl, P 〈 0. 001. Conclusions Our findings suggest that HDM is capable of inducing CD4^＋ CD25^＋ T cells recruitment into non-acute mild allergic asthmatic airways.

关 键 词：粉尘螨 CD4^+CD25^+T细胞 过敏性哮喘 

分 类 号：R686[医药卫生—骨科学]