c-erbB_2在大鼠卵巢中的表达及其在原始卵泡生长启动中的作用  

The expression of proto-oncogene c-erbB_2 and its role in the initiation of primordial follicle growth in rat ovary

作　　者：徐良全[1] 许爱霞[1] 黄健[1] 陈蔚云[1] 郑月慧[1] 

机构地区：[1]南昌大学医学院生殖研究所,南昌330006

出　　处：《生理学报》2009年第5期424-430,共7页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.39260035)

摘　　要：本文旨在研究原癌基因c-erbB2在大鼠卵巢的表达,及其在原始卵泡启动过程中的作用。我们首先采用RT-PCR方法证实大鼠卵巢中有c-erbB2的表达,再体外合成其RNA小干扰片段(small interference RNA,siRNA),并用脂质体(Metafectene)介导转染体外培养的新生大鼠卵巢,在引发原癌基因c-erbB2表达沉默或抑制的情况下,观察原始卵泡的生长启动情况,以及表皮生长因子(epidermal growthfactor,EGF)在此过程中的作用是否发生变化。结果显示:将c-erbB2siRNA转染原始卵泡后,c-erbB2 mRNA水平明显下调(P<0.05),三对siRNA抑制率分别为49.6%、46.7%、82.6%,同时其蛋白表达也明显下调(P<0.01)。通过siRNA抑制原癌基因c-erbB2的表达,原始卵泡的启动、生长受到抑制;EGF可以促进原始卵泡的启动生长,但此作用可以被c-erbB2 siRNA阻断。以上结果提示,c-erbB2参与原始卵泡启动生长过程的调控,并介导EGF促进原始卵泡启动生长的作用。Little is known about the factors that control the initiation of growth of primordial follicles. The objective of the present study was to investigate the effect of c-erbB2 on the onset of primordial follicle development, and whether c-erbB2 mediates the effect of epidermal growth factor （EGF） in this process. We synthesized three pairs of siRNAs targeting the c-erbB2 mRNA and transferred them into the newborn rat ovary cultured in vitro with Metafectene. After siRNAs transfection, the efficiency of siRNAs was tested by examining c-erbB2 mRNA and protein levels. The level of c-erbB2 mRNA was reduced by 49.6%, 46.7% and 82.6% respectively after transfecting siRNA 1, siRNA2 and siRNA3, and the level of ErbB2 protein also reduced remarkably after siRNA3 transfection. c-erbB2 siRNA3 significantly inhibited the primordial follicle initiation and development; EGF augmented primordial follicles formation, but the effect was abolished by c-erbB2 siRNA3. All of these results suggest that c-erbB2 plays an important role in primordial follicle development and folliculogenesis initiation, and mediates the effect of EGF on primordial follicle development.

关 键 词：卵巢 原始卵泡 C-ERBB2 表皮生长因子 RNA干扰 

分 类 号：Q343[生物学—遗传学]