扩增片段多态性分析鲍曼不动杆菌的同源性  

Molecular typing for Acinetobacter baumannii by amplified fragment length polymorphism

作　　者：张顺[1] 蔡挺[2] 许小敏[1] 陈琳[1] 余静[1] 

机构地区：[1]宁波市第二医院临床研究室,315010 [2]宁波市医疗中心李惠利医院重症监护病房,315012

出　　处：《国际流行病学传染病学杂志》2009年第5期308-310,共3页International Journal of Epidemiology and Infectious Disease

摘　　要：目的 采用扩增片段长度多态性（AFLP）技术，分析鲍曼不动杆菌（鲍氏不动杆菌）菌株亲缘性。方法建立AFLP分型平台研究鲍曼不动杆菌基因型，对检测结果作聚合分析并分析菌株亲缘性。结果经AFLP分型，27株鲍曼不动杆菌可分为2个亚型：AFLPⅠ和AFLPⅡ。结论AFLP分型可用于鲍曼不动杆菌的基因分型，有助于观察其耐药性变化和监测院内感染。Objective To analyse the relation of Acinetobacter baumannii in Ningbo No.2 Hospital by amplified fragment length polymorphism（AFLP）. Methods The genetype of 27 A. baumannii were analyzed by AFLP. The relation was analyzed with cluster analysis methods. Results 27 strains of A. baumannii were divided into two groups by AFLP method. Conclusions AFLP has more clinical practice to study the change of the multi-drug resistance of A.baumannii and the prevalence of A. baumannii in nosocomial infection.

关 键 词：鲍氏不动杆菌 扩增片段长度多态性 耐药基因 院内感染 

分 类 号：R450[医药卫生—治疗学]