阻断颈淋巴加重实验性蛛网膜下腔出血软脑膜脑微循环异常  

Blockade of Cervical Lymphatic Deteriorate Pial Microcirculation of Rat with Experimental Subarachnoid Hemorrhage

作　　者：王轩[1] 贾丽丽[1] 孙保亮[2,3] 杨明峰[2,3] 张颜波[2,3] 袁慧[2,3] 李汶霞[2,3] 

机构地区：[1]章丘市人民医院神经内科,山东章丘250200 [2]山东省高校脑微循环重点实验室(泰山医学院) [3]泰山医学院附属医院神经内科

出　　处：《中国微循环》2009年第5期348-350,共3页Journal of Chinese Microcirculation

基　　金：国家自然科学基金资助项目(No.30570651;No.30670724;No.30770759);山东省教育厅科技计划项目(J05L10);山东省中医药科技发展计划项目(2005-231);山东省卫生系统高层次人才基金资助

摘　　要：目的探讨脑淋巴引流阻滞对蛛网膜下腔出血(SAH)后软脑膜微循环的影响。方法选用成年健康Wistar大鼠,随机分为正常对照组(A组)、SAH组(B组)、颈淋巴阻断+SAH组(CLB+SAH组,C组)。采用枕大池二次注血法建立SAH模型。监测动脉血压和血气,在体观察脑膜微循环血流及管径变化。结果各组动物生理指标相对恒定。B、C微动脉、微静脉明显痉挛,而C组更为严重;A组软脑膜微循环血供丰富,血流快速无凝集。B、C组微循环血流多呈泥沙样流动,甚至可见血流郁滞、摆动,以C组更为显著。结论脑淋巴引流途径在SAH后脑微循环障碍和脑损伤中可能起重要作用。Objective To determine the possible influence of cerebral lymphatic pathway blockage on microcirculation after subaraehnoid hemorrhage （SAH）. Methods Healthy adult Wistar rats were divided into normal control group（ group A）, SAH group （group B）, and SAH plus cervical lymphatic blockage （CLB） group （ group C）. The SAH models were established by twice injection of autologous arterial blood into cisterna magna. The CLB models were established by occlusion of cervical lymphatic vessels and removal of lymph nodes. The arterial blood gas and blood pressure were monitored. The changes of pial microcirculation were observed in vivo. Results The physiological parameters maintained relatively constant in each group. In group B and group C, spasm of arterioles and veinules was detected, and the spare in group C was more severe than that in group B. In group A, abundant blood flow in pial microcireulation was documented. However, the blood flow became abnormal eharacteristiced by silted, wobbly flow and stasis. The abnormality of pial microcirculation was more obvious in group C than that in group B. Conclusion The cerebral lymphatic drainage pathway may play an important role in the development of cerebral microcireulation turbulences and cerebral injury after SAH.

关 键 词：蛛网膜下腔出血 脑损伤 脑淋巴引流 微循环 

分 类 号：R331.35[医药卫生—人体生理学] R743.35[医药卫生—基础医学]