红球菌Rhodococcussp-1对地下水中Mn(Ⅱ)生物去除特性的研究  

Study on the groundwater Mn(Ⅱ) biological removal characteristics of Rhodococcus sp-1

作　　者：段晓东[1] 宋立新[1] 杨宏[1] 吕鑑[1] 张杰[1] 

机构地区：[1]北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京100022

出　　处：《给水排水》2009年第11期146-149,共4页Water & Wastewater Engineering

基　　金：国家高技术研究发展计划"863"项目(2006AA06Z308)

摘　　要：利用传统的细菌分离方法,从地下水生物除锰滤池中分离、纯化出一种细菌,经LBB法检测其具有Mn(Ⅱ)氧化能力,利用Sherlock.细菌鉴定系统初步确定其属于红球菌属(Rhodococcussp.)。对红球菌Rhodococcus sp-1菌株的Mn(Ⅱ)生物去除特性进行研究,结果表明:Rhodococcussp-1菌株在接种20 h左右达到生长稳定期,Mn(Ⅱ)的去除量趋于稳定,可达到12 mg/L,具有很强的地下水Mn(Ⅱ)生物去除能力;同时Rhodococcus sp-1菌株的生长可以改变其培养环境的pH,使pH升高。为锰生物氧化机理和特性的研究提供了优势菌株,同时为后续的锰氧化细菌固定化和酶学研究奠定了基础。In this research, a kind of bacteria had been separated and purified from the groundwater Manganese removal bio-fiher by traditional microbiology method. These bacteria were proven to have the Mn（Ⅱ） oxidizing ability by the LBB method and belong to Rhodococcus sp. by the MIDI Sherlock bacteria identification system. The study on the Mn（Ⅱ） removal characteristics of Rhodococcus sp-1 showed that： the growth of Rhodococcus sp-1 reached the stabilization period after inoculation 20 h, and the stable Mn（Ⅱ） removal amount was 12 rag/L, which meant it had strong groundwater Mn（Ⅱ） biological removal ability; meanwhile, the growth of Rhodococcus sp-1 could increase the environmental pH value. This study offered the dominating bacterial strain for the study on Manganese oxidation mechanisms and characteristics and built ground for the further research on the Manganese oxidizing bacteria immobilization and enzymology.

关 键 词：红球菌 生物除锰 地下水 Mn(Ⅱ)生物去除特性 

分 类 号：X703[环境科学与工程—环境工程] TM911[电气工程—电力电子与电力传动]