鲍氏不动杆菌多药耐药与Ⅰ类整合子相关性的研究  

Multidrug Resistance of Acinetobacter baumannii and Its Relateness of Class Ⅰ Integron:A Correletive Study

作　　者：丁群力[1] 刘平[2] 叶惠芬[2] 

机构地区：[1]宁波大学医学院附属医院呼吸内科,浙江宁波315020 [2]广州医学院附属市第一人民医院中心ICU,广东广州510182

出　　处：《中华医院感染学杂志》2009年第22期3001-3003,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨鲍氏不动杆菌的多药耐药与Ⅰ类整合子的相关性。方法收集39株医院感染多药耐药鲍氏不动杆菌株及41株非多药耐药鲍氏不动杆菌株,以纸片扩散法敏感试验(K-B法)检测其耐药表型,以聚合酶链反应(PCR)方法检测Ⅰ类整合子的分布情况,分析Ⅰ类整合子与细菌耐药表型的相关性。结果鲍氏不动杆菌对多种抗菌药物的耐药率均较高,除对亚胺培南、头孢哌酮/舒巴坦、环丙沙星、阿米卡星、哌拉西林/他唑巴坦敏感率较高外,对其他抗菌药物的耐药率均>40.0%;多药耐药鲍氏不动杆菌中Ⅰ类整合子阳性率为82.1%,非多药耐药菌株阳性率为26.8%,多药耐药菌与非多药耐药菌株Ⅰ类整合子阳性率差异有统计学意义(2χ=24.6,P<0.01);比较研究Ⅰ类整合子阳性菌株耐药表型与Ⅰ类整合子阴性菌株耐药表型发现,除了对亚胺培南敏感率均较高,对氨曲南均耐药,两者耐药率之间差异无统计学意义外,对其他抗菌药物耐药率差异均有统计学意义(P<0.05)。结论Ⅰ类整合子的表达与鲍氏不动杆菌多药耐药密切相关。OBJECTIVE To explore the relationship between multidrug resistance of Acinetobacter baumannii （ABA） and its class I integron. METHODS In this study, 39 multidrug resistant strains and 41 non-rnultidrug resistant strains of ABA were collected. The improved Kirby-Bauer was employed to check the collected strains＇ drug-resistant phenotype; PCR was administrated to detect the distribution of class I integron, and their relationship was also analyzed. RESULTS Results showed that ABA＇s drug-resistance rate to the most antibiotics was high. imipenem, cefoperazone-sulbactam, ABA was sensitive to ciprofloxacin, amikacin and Piperacillin- tazobactam, however, ABA＇s drug resistance rates to other antibiotics were all over 40%. It revealed that ABA＇s drug-resistance rate was high. The study indicated that the positive rate of Class I integron in multidrug resistant ABA was as hight as 82.1 % （32/39）. The positive rate of non-multidrug resistant strains was 26.8% （11/41）, and the differences were statistically significant（Х^2 = 24.6, P〈0.01）. The study also compared the drug-resistance rate of ABA strains with Class I integron and that of ABA strains without Class I Integron. The result indicated that ABA stains with and without Class I integron were both sensitive to imipenem and resistant to aztreonam, and there were no differences between two groups. Drug resistance rate of ABA strains with and without Class I integron to all others showed statistically significant differences （P〈0.05）. CONCLUSIONS It reveals the expression of class 1 integron is closely related with the multidrug resistance of ABA.

关 键 词：I类整合子 鲍氏不动杆菌 多药耐药 

分 类 号：R378[医药卫生—病原生物学]