聚合酶链反应在检测脑脊液病原菌中的应用  

Application of Polymerase Chain Reaction in Detection of Pathogens in Cerebrospinal Fluid

作　　者：张仙森[1] 喻长法[1] 段达荣[1] 任应鹏[1] 阮荣华[1] 应小明[1] 

机构地区：[1]台州市第一人民医院检验科,浙江台州318020

出　　处：《中华医院感染学杂志》2009年第22期3139-3141,共3页Chinese Journal of Nosocomiology

基　　金：浙江省黄岩科技局资助(07040)

摘　　要：目的应用聚合酶链反应(PCR)建立快速检查方法来检测脑脊液中是否含有病原菌。方法对138例脑脊液标本,以病原菌16S rRNA基因为靶序列,采用1对通用引物对其进行PCR扩增检测,并同时作细菌培养。结果138例脑脊液标本中,PCR方法检测出55例,阳性率为39.86%,培养法检出24例,阳性率为17.39%,PCR方法检出率明显高于培养法(P<0.01)。结论该方法具有快速、敏感、特异、准确等优点,是一种可靠的实验手段。OBJECTIVE To set up a quick method to detect pathogens in cerebrospinal fluid. METHODS The method of polymerase chain reaction （PCR）, using a pair of universal primers targeted at the 168 rRNA gene, was adopted to amplify the DNA of bacterium. 138 clinical specimens obtained from patients were examined by PCR method and bacterial culture method. RESULTS The positive rate was 39.86% of PCR and 17.39% of culture,the outcomes had statistical significance （P〈0.01）. CONCLUSIONS The method of PCR is fast, sensitive,specific and accurate for detecting pathogens.

关 键 词：聚合酶链反应 脑脊液 病原菌 

分 类 号：R446[医药卫生—诊断学]