人乳头状瘤病毒E6/E7mRNA检测宫颈高度病变的临床评价  

Detection of HPV E6/E7 mRNA Transcript in High-grade Cervical Lesion:An Evaluation of Diagnostic Accuracy

作　　者：卢文波[1] 姜智南[2] 陈顺美[3] 覃世榕[1] 赵玲军[3] 杨海涛[4] 施丹华[1] 陈雪梅[1] 

机构地区：[1]宁波市妇女儿童医院检验科,浙江宁波315012 [2]宁波市妇女儿童医院肿瘤科,浙江宁波315012 [3]宁波市妇女儿童医院妇产科,浙江宁波315012 [4]宁波市妇女儿童医院病理科,浙江宁波315012

出　　处：《中华医院感染学杂志》2009年第22期3145-3147,共3页Chinese Journal of Nosocomiology

基　　金：宁波市科技局资助项目(2008A610087)

摘　　要：目的评价人乳头状瘤病毒(HPV)E6/E7 mRNA检测宫颈高度病变的效能。方法用PreTectTMHPV-Proofer检测法检测65例妇女宫颈上皮细胞HPV E6/E7 mRNA转录情况,以液基细胞学检测(LBC)结果为标准评价HPV E6/E7 mRNA检测宫颈高度病变[不能除外高度不典型鳞状细胞(≥ASC-H)]的效能。结果高度组(≥ASC-H组)HPV E6/E7 mRNA转录阳性率(63.6%)高于低度组(<ASC-H组)(28.6%),两组间差异有统计学意义(2χ=5.71,P<0.05);HPV E6/E7 mRNA检测宫颈高度病变的敏感性为63.6%,特异性为77.8%。结论宫颈高度病变与HPV E6/E7 mRNA的转录有关,在宫颈癌筛查中检测HPV E6/E7 mRNA的转录具有潜在价值。OBJECTIVE To evaluate the diagnostic accuracy of HPV E6/E7 mRNA detection in high-grade cervical lesion. METHODS Sixty-five women were enrolled and their cervical squamous cell specimens were collected for detecting the transcription of HPV E6/E7 mRNA by PreTectTM HPV-Proofer, and the diagnostic accuracy of HPV E6/E7 mRNA detection in high-grade cervical lesion atypical squamous cells high grade （≥ASC-H） could not exclude high-grade squamous intraepithelial lesion （HSIL） was analyzed using liquid based cytology （LBC） result as standard. RESULTS 28.6% women were positive for HPV E6/E7 mRNA transcript in 〈ASC-H group and 63.6% women were positive for HPV E6/E7 mRNA transcript in ≥ASC-H group, there was significant difference between them （ Х^2 = 5.71, P〈 0. 05）. The sensitivity and specificity were 63. 6% and 77. 8%, respectively. CONCLUSIONS There is significant correlation between HPV E6/E7 mRNA transcript and high- grade cervical lesion. The detection of HPV E6/E7 mRNA transcript has potential value in cervical cancer screening.

关 键 词：人乳头状瘤病毒 宫颈高度病变 E6/E7 MRNA转录 敏感性 特异性 

分 类 号：R373.9[医药卫生—病原生物学]