不同激素种类和配比对秤锤树愈伤组织诱导的影响研究  

Research on the Effects of Different Hormones and Their Combinations on the Callus Induction of Sinojackia xylocarpa

作　　者：曹昆[1,2] 李霞[2] 

机构地区：[1]南京农业大学生命科学院,江苏南京210095 [2]江苏省农业科学院粮食作物研究所江苏省优质水稻工程技术研究中心,江苏南京210014

出　　处：《安徽农业科学》2009年第32期15694-15696,15806,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30871459);江苏省农业科技创新基金(CX[07]603);江苏省农业科学院基金项目(6510707;6110704)

摘　　要：[目的]为秤锤树的组培快繁提供依据。[方法]以WPM培养基为基本培养基,秤锤树叶片为外植体,比较了不同激素及其组合对秤锤树愈伤组织诱导的影响。[结果]不同浓度2,4-D诱导出的愈伤组织呈褐色透明的水渍状,放置约40d后逐渐褐化并坏死;2.0mg/L2,4-D和不同浓度KT的激素组合诱导出的愈伤组织呈乳白色的致密颗粒状或团块状。单独使用6-BA未诱导出愈伤组织,6-BA和NAA的激素组合诱导出了愈伤组织。当6-BA添加量为1.0mg/L时,出愈率随着NAA添加量的增加而提高。单独使用TDZ未诱导出愈伤组织,TDZ和NAA的不同浓度组合的出愈率分别为46.5%、37.1%、25.4%、24.5%和11.7%,诱导出的愈伤组织呈绿色、颗粒状、小而致密。[结论]只有激素组合2,4-D2.0mg/L+KT0.25～2.00mg/L才能诱导出致密、颗粒状、生长快的愈伤组织。[ Objective ] The study aimed to provide basis for the tissue culture and rapid propagation for Sinojackia xylocarpa. [ Method ] With WPM medium as basic culture medium, and S. xylocarpa leaves as explant, the effects of different hormones and their combinations on induction for S. xylocarpa callus were compared. [ Result] The callus induced out by 2,4-D with different eoncn, presented brown transparent watery stain, and was browning gradually and necrotic after placed about 40 d. The callus induced by hormone combination of 2,4-D of 2.0 mg/L and KT with different eonen, showed milk-white compact grainy or block mass state. The callus was not induced by using 6-BA alone, and the callus was induced by hormone combination of 6-BA and NAA. When the addition amount of 6-BA was 1.00 mg/L, the callus rate was enhanced along with the increment of addition amount of NAA. The callus rates of combination of TDZ and NAA with different eonen. were 46.5%, 37.1% , 25.4%, 24.5% and 11.7% resp. , the induced callus presented green and grainy state, small and compact. [Conclusionl The dense and grainy callus with quick growth could be induced only by the hormone combination of 2,4-D 2.0 mg/L ＋ KT 0.25 - 2.00 mg/L.

关 键 词：秤锤树 愈伤组织 诱导 

分 类 号：S722.37[农业科学—林木遗传育种]