应用等密度梯度离心法提纯传染性支气管炎病毒  

PURIFICATION OF INFECTIOUS BRONCHITIS VIRUS BY ISOPYCNIC CENTRIFUGATION ON SUCROSE GRADIENTS

作　　者：陈丽君[1] 王英[1] 胡建华[1] 王建荣[1] 康尧[2] 王阿凤[2] 赵建华[2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市农业科学院生物测试中心,上海201106

出　　处：《上海农业学报》1998年第3期93-95,共3页Acta Agriculturae Shanghai

基　　金：上海市农业科学院青年科技基金

摘　　要：采用分段取样，测OD260，及电镜观察等方法，对用等密度梯度离心法提纯的传染性支气管炎病毒粒子在蔗糖溶液中的病毒富集带和纤突丢失程度进行了分析和总结。提出梯度离心后30％～50％区带蔗糖溶液为较理想的取样位置。The concentrated position and the losing of spike of infectious bronchitis virus particles which were purified by isopycnic centrifugations on sucrose gradients were analysed by reading at wavelengths of 260 urn and observing under electron microscope,the band of 30%-- 50% in sucrose gradients was thought as the ideal position for separating virus after isopycnic centifugation.

关 键 词：传染性 支气管炎病毒 等密度梯度离心 电镜观察 

分 类 号：S856.3[农业科学—临床兽医学]