川芎嗪对血虚证小鼠骨髓细胞IL-4R和IL-7R基因表达的作用  

Effect of Ligustrazine on IL-4R and IL-7R Gene Expression in Bone Marrow Cell of Blood Deficency Mice Induced by Irradiation

作　　者：郭平[1] 郭霞[2] 

机构地区：[1]山东中医药大学生化教研室,济南250355 [2]山东中医药大学附属医院,济南250011

出　　处：《中国医药导刊》2009年第11期1895-1896,共2页Chinese Journal of Medicinal Guide

基　　金：国家自然科学基金资助项目(30271617)

摘　　要：目的:考察川芎嗪对辐射致血虚证小鼠骨髓细胞IL-4R和IL-7R基因表达的影响,为阐明川芎嗪的补血机制提供依据。方法:采用3.5Gy^(60)Coγ射线全身1次性照射制备小鼠血虚证模型;骨髓集落培养计数CFU-GM、CFU-E、BFU-E、CFU-mix;RT-PCR检测骨髓IL-4R和IL-7R基因表达。结果:川芎嗪显著升高放射线致血虚证小鼠骨髓CFU-GM、CFU-E、BFU-E、CFU-mix数量,上调骨髓细胞IL-4R和IL-7R基因表达。结论:川芎嗪促进骨髓细胞IL-4R和IL-7R基因表达可能是其升血和调节免疫功能的机制之一。Objective：To observe the effect of ligustrazine on IL-4R and IL-7R gene expression in bone marrow cell of blood deficiency mice induced by radiation and to provide the foandation for explaining the blood enriching mechanism of ligustrazine.Methods：The blood deficiency mice were induced by using a single dose of 3.5 Gy 60Co γ radiation.Myeloid colony cell was cultured and the number of CFU- GM,CFU-E,BFU-E and CFU-mix were counted.IL-4R and IL-7R gene expression in bone marrow were measured by RT-PCR.Results： Ligustrazine can raise significantly the number of CFU-GM,CFU-E,BFU-E and CFU-mix in bone marrow of blood deficiency mice and upregulate IL-4R and IL-TR gene expression of bone marrow cett.Conclusions：Ligustrazine can promote IL-4R and IL-7R gene expression in bone marrow cells,which probably is one of the mechanisms of blood proliferation and immune regulation.

关 键 词：川芎嗪 辐射 血虚证 造血祖细胞 白分素受体 

分 类 号：R285.5[医药卫生—中药学]