HPLC测定舒肝降逆颗粒中芍药苷含量  

Quantitative Determination of Paeoniflorin in Shugan Jiangni Granules by HPLC

作　　者：宋治荣[1] 王兰霞 李士博[2] 

机构地区：[1]甘肃中医学院,兰州730000 [2]甘肃药品检验所,兰州730000

出　　处：《中国医药导刊》2009年第11期1985-1986,共2页Chinese Journal of Medicinal Guide

摘　　要：目的:建立舒肝降逆颗粒中芍药苷的HPLC测定方法。方法:采用Phenomenex C_(18)柱(150mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸液(20:80);流速1.0ml/min;检测波长为230nm;柱温30℃。结果:芍药苷在0.3984～1.992μg范围内线性关系良好(r=0.9999),平均加样回收率为100.21%,RSD为1.30%。结论:本方法简便快捷,结果准确,可用于控制舒肝降逆颗粒的质量。Objective：To establisb HPLC method for determination of paeoniflorin in Shugan Jiangni particles.Methods：Phenomenex C18 column（150mm×4.6mm,5μm）was used in HPLC with Acetonitrile-0.1% Phosphoric acid（20：80） as mobile phase.The flow rate was 1.0ml/min.Detectionwavelength was 230nm.Column ternperature was at 30℃.Results：The good linear range of Paconiflorin was 0.3984-1.992μg（r=0.9999）,average recovery was 100.21% with RSD of 1.30%.Conclusions：The method was simple and quick,accurate and can be used to control the quality of Shugan jiangni particle.

关 键 词：舒肝降逆颗粒 芍药苷 高效液相色谱 

分 类 号：R286.0[医药卫生—中药学]