不同密度下玉米DH群体果穗性状的QTL定位分析  

QTL mapping for ear traits under different densities using DH population of maize

作　　者：张建华[1] 刘志增[1] 祝丽英[1] 黄亚群[1] 陈景堂[1] 高慧[1] 赵永锋[1] 

机构地区：[1]河北农业大学农学院,河北保定071001

出　　处：《河北农业大学学报》2009年第5期1-6,17,共7页Journal of Hebei Agricultural University

基　　金：河北省十一五科技攻关(20061148001);河北农业大学科技将帅计划

摘　　要：试验利用由79个DH系组成的‘农单5号’DH群体,分别在6万株/hm2和13.5万株/hm22个密度、2次重复条件下,调查了玉米穗长、秃尖长、穗粗、穗行数、行粒数、粒长、粒宽和粒厚等穗部性状,利用在双亲间存在差异且均匀分布于玉米10条染色体上的160对SSR标记对该群体进行遗传作图,并采用复合区间作图法对上述性状进行QTL分析。结果表明：在2个密度条件下共检测出30个QTL位点,单个QTL所解释的表型变异在3.26%~31.59%之间,QTL与环境之间存在复杂的互作关系。在2个密度水平下分别检测到的qEL2a（umc1165-umc1261）与qEL2b（umc1165-umc1261）可能是同一位点,qRN4a（umc2135-bnlg292a）、qRN4b（bn-lg292a-bnlg292b）和qED4（bnlg292a-bnlg292b）也很可能是同一位点。在4.08 bin区间存在着控制穗粗或穗行数的重要基因,而该基因位于umc2135-bnlg292b标记区间。A total of 79 DH lines derived from Nongdan 5 were used to test QTL which controlled ear traits under two densities （60 000 plants/hm2 and 135 000 plants/hm2） and two replications, respectively. Genetic map of the population was constructed by using 160 polymor- phic SSR makers on the 10 chromosomes. The composite interval mapping method was em- ployed to analyze the QTLs for maize ear traits such as ear length, barren tip length, ear diameter, ear row number, kernel number per row, kernel length, kernel width and kernel thickness. A total of 30 QTLs were detected under the two densities, the phenotypic variation illus- trated by individual QTL ranged from 3.26% to 31.59%,with complicated interaction between QTL and environments. The QTL qEL2a （umc1165 - umc1261） and qEL2b （umcl165 - umc1261） detected under two densities should be the same loci, and qRN4a, qRN4b and qED4 probably are on a common site. and ear row numbers . The results in the bin 4. suggested that there was an important gene for ear diameter 08, and this gene was located in the umc2135 -bnlg292b interval.

关 键 词：玉米 DH群体 穗部性状 QTL 

分 类 号：S513[农业科学—作物学]