丰富环境干预对缺氧缺血性脑损伤新生大鼠海马神经元影响的研究  

Study of effect of enriched environmental inteference on neurons in hippocampus of HIBD neonatal rats

作　　者：于若谷[1] 李刚[1] 钟晓云[1] 刘晓蓉[1] 

机构地区：[1]重庆市妇幼保健院,400013

出　　处：《重庆医学》2009年第22期2804-2805,2808,共3页Chongqing medicine

摘　　要：目的通过检测早期环境干预后缺氧缺血性脑损伤(HIBD)大鼠脑海马区存活神经元数目的改变,探讨早期环境干预对于促进缺血缺氧的脑组织恢复及神经网络重建的可能机制。方法7日龄Sprague-Dawley(SD)新生大鼠,采用Rice法建立HIBD模型,随机分为3组:干预组、非干预组及正常组,于建模后24h进行早期抚触和丰富环境干预,干预28d后,应用Morris水迷宫测试大鼠学习记忆能力,测量体质量,HE染色观察海马病理形态学改变,尼氏染色计数海马区存活神经元数目。结果HE染色见干预组患侧海马轻度异常;非干预组左侧海马明显异常;正常组左侧海马无异常。神经元尼氏染色计数显示,干预组患侧海马神经元存活数目明显多于非干预组(P<0.001),与正常组比较差异无统计学意义(P>0.05)。结论(1)丰富环境可减少海马区神经细胞的丢失,因此对HIBD新生鼠的神经元具有保护作用;(2)早期环境干预能显著改善HIBD大鼠的远期学习记忆功能,提示早期环境干预对保护远期学习记忆功能有重要意义。Objective To explore the possible mechanism of the recovery of hypoxic-ischemic brain damage（HIBD） and the reconstruction of the brain nervous net with early envinonmental inteference. Methods The Sprague-Dawley （SD） ratts models of HIBD were established by the method of Rice. Then the rats were divided into 3 groups at random：normal control group,hypoxicischemic（HI） group and enriched environment（EE） group. The early touch and enriched environment interference were administrated to the rats since 2d after HIBD. On 28d, Morris water maze was used to evaluate the learning and memory ability, also the weight of rats were measured. The HE stain and the Nissl stain were respectively employed to observe the morphopathologic changes and the number of live neurons in the left-sided hippocampus of the rats. Results The water maze scores of EE group in place navigation test and spatial probe test were much higher than that of HI group （P〈0. 001）, there was no significant difference compared EE group with the normal control group （P〉0.05）. The weight of EE group was much higher than that of HI group （P〈 0.01） ,there was no significant difference compared EE group with normal control group （P〉0.05）. HE stain displayed that the left-sided hippocampus of EE group was minimal abnormal with the slightly disorganized neurons and some cells lost; while the morphology of HI group was obviously abnormal with reduced cell layers; the morphology of normal control group was normal. The Nissl stain showed that the number of live neurons in the lefvsided hippocampus of EE group was much more than that of HI group （P〈0.01）. There was no significant difference compared EE group with normal control group （P〉0.05）. Conclusion （1） The decreased neuron damage in the injured side brain tissue of HIBD neonatal rats implies that the enriched environment could protect both the neuron and the axon. （2）The neonatal rats with HIBD have disturbance with the ability of long- term learning a

关 键 词：丰富环境 缺氧缺血 神经元 

分 类 号：R722.1[医药卫生—儿科]