骨髓源性干细胞在创面愈合中的募集作用  

Recruitment of bone marrow derived stem cells in derma wound repair

作　　者：孙士锦[1] 陈强[2] 杨在亮[3] 麻晓林[1] 张波[3] 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤中心,重庆400042 [2]第三军医大学大坪医院野战外科研究所麻醉科,重庆400042 [3]第三军医大学大坪医院野战外科研究所第四研究室,重庆400042

出　　处：《重庆医学》2009年第22期2819-2821,共3页Chongqing medicine

基　　金：国家重点基础研究发展规划(973)项目(2005CB522603)

摘　　要：目的观察BALB/C小鼠骨髓源性干细胞(BMSCs)在创面修复中的动员及募集作用,为进一步研究其向创伤部位募集机制进行前期探索。方法采用差异性贴壁法分离纯化BALB/C雄性小鼠BMSCs,取第3代BMSCs进行成脂、成骨诱导鉴定;另取第3代BMSCs用二醋酸羧基荧光素琥珀酰亚胺酯(CFDA-SE)标记后,经尾静脉注射到3.5Gy放射性损伤后的雌性小鼠体内,小鼠背部皮肤制作一直径为1.5cm的创面,建立骨髓移植嵌合模型,分别于伤后1、3、5、7、14d获取创面组织,观察创面愈合过程中是否募集有CFDA-SE标记的细胞。结果提取的BMSCs可以成功进行成骨、成脂诱导分化;骨髓移植后放射损伤实验动物全部存活,满足实验要求;皮肤创伤后创面组织中可以观察到CFDA-SE标记的细胞。结论皮肤创伤愈合过程中BM-SCs具有动员及向创面募集的作用,参与创面的愈合过程,提高这种募集作用有可能达到促进愈合的效果。Objecive To explore the mobilization and recruitment of bone marrow derived stem cells （BMSCs）in the derma wound repair for further study on the mechanisms of BMSCs in wound healing. Methods BMSCs were isolated and purified from BALB/C male mice bone marrow on the basis of the different adhenrence of cells to the wall of culture flask. The third generation of BMSCs was used for inducing osteogenesis and adipogenesis. And other third generation of BMSCs was marked with Carboxyfluorescein diacetate, succinimidyl ester（CFDA-SE）. Then, BALB/C female mice were transplanted with male BMSCs marked with CFDA-SE via the tail vein after 3.5Gy irradiation. The back skin wound was constructed in the bone marrow-chimeric mice in 4h after irradiation. Wound tissue was harvested in post-operation 1,3,5,7,14d in order to detect whether the CFDA-SE positive cells were exist in the derma wound. Results Osteogenesis and adipogenesis could be induced from BALB/C male mice BMSCs in vitro. The irradiated mice survived after BMSCs transplantation. The positive CFDA-SE cells were observed in the wound tissue after operation. Conclusion BMSCs exerts mobilization and recruitment in the wound area,as may be helpful for tissue repair.

关 键 词：干细胞 创伤和损伤 修复 募集 

分 类 号：R641[医药卫生—外科学]