EPCs促血管化组织工程骨修复大段骨缺损的早期组织学评价  

A histologic evaluation on vascularization of tissue engineering bone induced by endothelial progenitor cells(EPCs) for repairing large segmental defects in early period of bone healing

作　　者：吴雪晖[1] 谢肇[1] 罗飞[1] 许建中[1] 曾玲[1] 孙东[1] 

机构地区：[1]第三军医大学西南医院骨科,重庆400038

出　　处：《重庆医学》2009年第22期2839-2840,2843,I0003,共4页Chongqing medicine

基　　金：重庆市科委自然科学基金计划资助项目(CSTC;2007BB5044)

摘　　要：目的评价血管内皮祖细胞(EPCs)促血管化组织工程骨修复大段骨缺损的早期组织学结果。方法将来源于自体骨髓的EPCs与经成骨诱导的骨髓间充质干细胞(BMSCs)及脱钙骨基质(DBM)共同构建的促血管化组织工程骨修复兔桡骨大段骨缺损,通过光镜、扫描电镜、透射电镜等方法观察术后2、4周时骨缺损区的组织学改变。结果实验组(EPCs+BMSCs+DBM)软骨细胞和成骨细胞数量更多,功能更活跃,骨小梁更成熟,并可见较多梭形的血管内皮细胞,新生血管更丰富。结论EPCs促血管化组织工程骨能在骨愈合早期促进新生血管形成,并进一步促进成骨,加速骨愈合。Objective To observe the histologic evaluation on vascularization of tissue engineering bone induced by endothelial progenitor cells （EPCs） for repairing large segmental defects in early period of bone healing. Methods Vaseularization promoting tissue engineering bone constructed by EPCs derived from autogenous marrow,bone marrow derived mesenchymal stem cells （BMSCs） and demineralized bone matrix （DBM） were jointly applied to repair large segmental defects in rabbit radius; the histologic changes of defect areas were observed with light microscope, scanning electron microscope and transmission electron microscope etc. 2 weeks and 4 weeks postoperatively. Results There were more ehondrocytes and osteohlasts with more active functions in experimental group （EPCs-I-BMSCs＋DBM） and the bone trabecula was more mature; multiple spindle-shaped vascular endothelial cells could be observed and there were much more new blood vessels. Conculsion Vascularization of tissue engineering bone induced by EPCs can promote the neovascularization in early period of bone healing,and to further promote osteogenesis and speed up bone healing.

关 键 词：血管内皮祖细胞 组织工程骨 骨缺损 组织学 

分 类 号：R687[医药卫生—骨科学]