HPLC法测定藏药独一味中8-乙酰氧基山栀子苷甲酯的含量  

Determination of 8-O-acetyl-shanzhiside Methylester in Tibet Medicinal Lamiophlomis rotata by HPLC

作　　者：王娟[1] 李茂星[2] 李文斌[2] 樊鹏程[2] 张汝学[2] 贾正平[1,2] 

机构地区：[1]兰州大学药学院,兰州市730000 [2]兰州军区兰州总医院,兰州市730050

出　　处：《中国药房》2009年第33期2595-2596,共2页China Pharmacy

基　　金：全军医学科学技术研究"十一五"计划重大专项课题项目(06D005-4);"十一五"军队中医药研发推广专项课题项目(2006032001);甘肃省科技攻关计划项目(2GS054-A43-014-14)

摘　　要：目的:建立以高效液相色谱法测定藏药独一味中8-乙酰氧基山栀子苷甲酯含量的方法。方法:色谱柱为Symmetry C18(150mm×4.6mm,5μm),流动相为乙腈-水(15∶85),检测波长为234nm。结果:8-乙酰氧基山栀子苷甲酯的检测浓度在1.97～19.68μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.999 6);平均回收率为99.53%,RSD=1.73%(n=9)。结论:本方法简便、准确、专属性强,可为独一味药材品质评价及开发利用提供参考。OBJECTIVE： To establish an HPLC method for the determination of 8- O -acetyl- shanzhiside methylester in Tibet medicinal Lamiophlomis rotata .METHODS： HPLC analysis was performed on Symmetry C18（150 mm × 4.6 mm, 5 μm） column with the mixture of acetonitrile- water （15 ： 85） served as the mobile phase. The detection wavelength was set at 234 nm. RESULTS：The linear range of 8 O - acetyl shanzhiside methylester was 1.97-19.68μg .mL^-1（ r = 0.999 6） and its average recovery was 99.53% （RSD = 1.73%, n = 9）. CONCLUSION： The method is simple, accurate and specific, and it applies reference for quality evaluation and utilization of Tibet medicinal L. rotata.

关 键 词：高效液相色谱法 独一味 8-乙酰氧基山栀子苷甲酯 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]