乌药等4种中药饮片基因组DNA提取方法的改进  

Improvement in the genomic DNA extraction from 4 herbal pieces of Lindera aggregata Kosterm

作　　者：郭纯[1] 鲁耀邦[1] 聂媛媛[1] 唐金凤[1] 

机构地区：[1]湖南中医药大学药学院,长沙410208

出　　处：《中南药学》2009年第11期803-806,共4页Central South Pharmacy

基　　金：国家中医药管理局中医药留学回国人员择优资助项目(编号:2006LHR19)

摘　　要：目的从乌药、云木香、枳壳、槟榔中药饮片中提取可用于RAPD扩增的高质量基因组DNA。方法采用改良CTAB法粗提基因组DNA,部分基因组DNA再进一步用试剂盒纯化,采用琼脂糖凝胶电泳、紫外分光光度法及RAPD扩增效果进行检测并比较。结果饮片基因组DNA纯化后,以随机引物S28进行PCR扩增获得谱带更丰富、条带更清晰的RAPD条带。结论本文所建立的DNA提取方法可适合多数中药饮片基因组DNA的提取,并可进一步用于RAPD分析。Objective To extract high-quality genomic DNA which is used for RAPD amplification from herbal pieces such as Radix Linderae,Radix Aucklandiae,Fructus Aurantii and Semen Arecae.Methods Genomic DNA was extracted using improved CATB method,purified with kit and determined by agarose gel electrophoresis,UV spectrophotometry and RAPD amplification.Results The more profuse and clear RAPD bands were obtained with random primer S28 after DNA purification.Conclusion The present method is suitable for isolating genomic DNA from herbal pieces and for further analysis of RAPD.

关 键 词：乌药 云木香 枳壳 槟榔 DNA提取 

分 类 号：R284[医药卫生—中药学]