高效液相色谱法同时测定金嗓开音丸中绿原酸和连翘苷的含量  

Simultaneous determination of content of chlorogenic acid and phillyrin in Jinsangkaiyin pills by HPLC

作　　者：肖智朋 

机构地区：[1]怀化市第一人民医院,湖南怀化418000

出　　处：《中南药学》2009年第11期830-832,共3页Central South Pharmacy

摘　　要：目的建立HPLC法同时测定金嗓开音丸中绿原酸？连翘苷含量。方法采用HPLC法,色谱柱：DikmaDiamonsil C18柱,流动相：乙腈-0.085%磷酸,流速：1.0 mL.min-1,柱温：30℃,检测波长：280 nm。结果绿原酸进样量在0.037 04-0.370 4μg与峰面积线性关系良好,r=0.999 69,平均加样回收率为99.02%,RSD为0.82%（n=9）;连翘苷进样量在0.020 66-0.206 6μg与峰面积线性关系良好,r=0.999 48,平均加样回收率为98.81%,RSD为1.01%（n=9）。结论本法简便、准确、重现性好,能排除其他成分的干扰,可用于该产品质量控制。Objective To establish an HPLC method for the simultaneous determination of content of chlorogenic acid and phillyrin in Jinsangkaiyin pills.Methods C18 column was used with acetonitrile and 0.085% phosphoric acid solution as the mobile phase,the flow rate was 1.0 mL·min-1,the column temperature was 30 ℃ and the detection wavelength was 280 nm.Results The calibration curves were linear within 0.037 04～0.370 4 μg（r=0.999 69） for chlorogenic acid,and 0.020 66～0.206 6 μg（r=0.999 48） for phillyrin.The average recovery of them was 99.02%（RSD=0.82%,n=9） and 98.81%（RSD=1.01%,n=9）,respectively.Conclusion The method is specific,accurate and reproducible,and can be used for quality control.

关 键 词：高效液相色谱法 金嗓开音丸 绿原酸 连翘苷 

分 类 号：R917[医药卫生—药物分析学] R284.2[医药卫生—药学]