大肠杆菌K88ac,ST_1,LT_B基因融合  

Gene Fusion of Enterotoxigenic Escherichia coli K88ac Gene,ST_1 Gene and LT_B Gene

作　　者：许崇利[1,2] 许崇波[2] 黄江丽[1] 惠远峰[1] 

机构地区：[1]吉林化工学院环境与生物工程学院,吉林吉林132022 [2]大连大学医学院,辽宁大连116622

出　　处：《河北师范大学学报（自然科学版）》2009年第6期792-795,共4页Journal of Hebei Normal University：Natural Science

基　　金：吉林化工学院科研基金(2008)

摘　　要：利用PCR技术,从大肠杆菌C83902质粒中扩增出K88ac基因,再从含ST1-LTB融合基因的重组质粒pXSLT1中扩增出ST1-LTB融合基因,构建了含K88ac-ST1-LTB融合基因表达质粒的重组菌株BL21(DE3)(pET-28KSL).经酶切鉴定和序列测定证实,构建的重组质粒pET-28KSL含有K88ac-ST1-LTB融合基因,且基因序列和阅读框架正确.经ELISA检测,重组菌株表达的K88ac-ST1-LTB融合蛋白能够被ST1,LTB单抗和K88ac抗体识别,表明已成功构建了K88ac-ST-LT融合基因,并实现了在重组大肠杆菌中的表达.With the technology of PCR, K88ac gene and ST1-LTB fusion genes were amplified respectively from the plasmid of Escherichia coli C83902 and the recombinant plasmid pXSLTI. The recombinant plasmid pET28-KSL containing fusion gene K88ae-ST1-LTB was obtained and transformed into Escherichia coli BL21 （DE3）. The recombinant plasmid pET28-KSL contained the fusion gene K88ac-ST1-LTB which had correct sequence and ORF by identification of endonuclease-digesting and sequence analysis. The fusion proteins K88ac- ST1-LTB were expressed in the recombinant strain EL21 （DE3） （pET28-KSL）. By ELISA method, the expressed protein could be recognized by MAb of ST1,LTB and K88ac antibodies. The result indicated that the fusion gene K88ac-ST1-LTB of enterotoxigenic Escherichia coli was successfully constructed and expressed in Escherichia coli.

关 键 词：大肠杆菌 K88ac基因 ST1基因 LTB基因 基因融合 

分 类 号：S852[农业科学—基础兽医学] Q78[农业科学—兽医学]