左归丸与右归丸对EAE大鼠血浆Th1/Th2平衡的影响  

The Influences of Zuoguiwan and Youguiwan on Plasma Th1/Th2 in Rats with Experimental Autoimmune Encephalomyelitis

作　　者：周莉[1] 樊永平[1] 王蕾[2] 龚海洋[1] 叶明[1] 

机构地区：[1]首都医科大学附属北京天坛医院 [2]首都医科大学

出　　处：《中西医结合心脑血管病杂志》2009年第11期1304-1306,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然基金资助项目(No.3064069);北京市自然科学基金(No.7062022);北京市中医药重点学科基金(No.2004京中重Ⅳ14)资助项目

摘　　要：目的探讨左归丸、右归丸对实验性自身免疫性脑脊髓炎(experi mental autoi mmune encephalomyelitis,EAE)大鼠病程及其血浆Th1/Th2(IFN-γ/IL-4)的影响。方法用MBP免疫Lewis大鼠诱导EAE模型,随机分为模型组、激素组、左归丸组、右归丸组及正常对照组,研究各组间发病率、死亡率、神经功能评分的差异,并分别在急性期(免疫后第15天)和缓解期(免疫后第27天)采集血样,检测血浆细胞因子水平。结果右归丸组发病率低于其他各处理组(P<0.05);急性期神经功能评分左归丸组和右归丸组均低于模型组和激素组(P<0.05);急性期左归丸组血浆Th1/Th2水平低于模型组(P<0.05);缓解期模型组、激素组Th1/Th2水平均低于对照组(P<0.05),左归丸组、右归丸组Th1/Th2水平均高于模型组和激素组(P<0.05),右归丸组Th1/Th2水平高于对照组(P<0.05);模型组Th1/Th2水平在缓解期下降(P<0.01),左归丸组和右归丸组Th1/Th2水平均在缓解期升高(P<0.01)。结论右归丸可降低EAE发病率;左归丸和右归丸均能减轻EAE疾病严重程度,治疗效果优于醋酸泼尼松;左归丸可下调EAE急性期血浆Th1/Th2水平;左归丸和右归丸均能上调EAE缓解期血浆Th1/Th2水平。Objective To investigate the effects of Zuoguiwan and Youguiwan on the level of plasma Th1/Th2 （interferon - γ/interleukin-4） in Lewis rats with experimental autoimmune encephalomyelitis（EAE）. Methods Lewis rats were immunized with the myelin Lasic protein （MBP）. Rats were randomly divided into five groups： Nomal group, EAE group, prednisone group, Zuoguiwan group and Youguiwan group after post immunization（H）. Rats were monitored daily PI, the clinical score of the degree of paralysis were recorded. On 15th day（acute stage） and 27th day（remitting stage） after PI, rats were killed and the peripheral blood were re- moved for cytokines examination. Results The incidence of EAE in Youguiwan group was significantly lower than that in other groups（P〈0. 05）. The paralysis score in Zuoguiwan group and Youguiwan group was lower than that in EAE group and prednisone group（P〈0.05）. On 15th day after PI,the level of plasma Th1/Th2 in Zuoguiwan group was lower than that in EAE group（P〈0. 05）. On 27th day after PI,the level of plasma Th1/Th2 in EAE group and prednisone group was lower than that in Nomal group（P〈0.01） ; and the level of plasma Th1/Th2 in Zuoguiwan group and Youguiwan group was higher than that in EAE group and prednisone group （P〈0.01）. The level of plasma Th1/Th2 in Youguiwan group was higher than that in Nomal group（P〈0.01）. On 27th day after PI, the level of plasma Th1/Th2 in EAE group was decreased（P〈0.01）, while the level of plasma Th1/Th2 Zuoguiwan group and Youguiwan group in was increased（P〈0.01）. Conclusion Youguiwan could decrease the incidence of EAE. The therapeatie efficacy of Zuoguiwan and Youguiwan were better than that of prednisone in alleviating clinical signs. Zuoguiwan could downregulate the plasma Th1/Th2 level in acute stage of EAE. Zuoguiwan and Youguiwan could upregulate the plasma Th1/Th2 level in remitting stage of EAE.

关 键 词：自身免疫性疾病 实验性自身免疫性脑脊髓炎 细胞因子 左归丸 右归丸 

分 类 号：R285.5[医药卫生—中药学]