弓形虫感染对妊娠中期孕鼠血清中胎盘激素含量的影响  

Effects of serum placental hormones in BALB/c mice infected with Toxoplasma gondii during the second trimester

作　　者：岳慧萍[1] 饶华祥[2] 侯玉英[1] 赫燕侠[1] 赵云鹤[1] 刘红丽[1] 徐秀文[1] 张杰[1] 侯丽萍 

机构地区：[1]山西医科大学寄生虫学教研室,山西太原030001 [2]青海省疾病预防控制中心传染病预防控制所,青海西宁810007 [3]山西省煤炭中心医院,山西太原030007

出　　处：《中国病原生物学杂志》2009年第10期763-766,共4页Journal of Pathogen Biology

基　　金：山西省科技研究开发项目(No.200613015);山西省自然基金项目(2009年立项);山西医科大学创新基金项目(2009年立项)

摘　　要：目的探讨妊娠中期弓形虫感染对BALB/c孕鼠血清绒毛膜促性腺激素(CG)、胎盘催乳素(PL)和雌三醇(E3)含量的影响。方法在妊娠第8 d,采用腹腔注射法建立弓形虫感染的BALB/c小鼠动物模型,于妊娠第12、14、16、18d分批摘眼球取血,处死孕鼠,采用放射免疫分析法(RIA)测定孕鼠血清CG、PL和E3含量。结果实验组和正常对照组孕鼠血清CG含量均随妊娠天数的增加而降低,PL和E3含量均随妊娠天数的增加而升高(CG:F对照组=19.306,F实验组=57.620;PL:F对照组=17.449,F实验组=46.720;E3:F对照组=239.546,F实验组=22.841,P均<0.01);妊娠第16 d和第18 d时3种激素含量实验组与正常对照组比较差异有统计学意义(CG:t16=4.351,t18=13.367;PL:t16=4.034,t18=2.875;E3:t16=4.265,t18=11.499,P均<0.01)。结论妊娠中期弓形虫感染孕鼠血清中胎盘激素含量较正常对照组显著降低,这可能是虫体感染所致的胎盘组织病理损伤和滋养层细胞凋亡增加的结果,也可能是感染所致胎儿宫内发育迟缓、死产、流产等不良妊娠发生的原因。Objective To investigate the concentration of serum chorionic gonadotropin （CG）, placental lactogen （PL）, and estriol （E3） in BALB/c mice infected with Toxoplasma gondii during the second trimester. Methods An animal model was established by intraperitoneal injection （i. p. ） of T. gondii on the 8^th day of pregnancy, and pregnant mice were sacrificed on the 12^th , 14^th , 16^th , and 18^th day of pregnancy. The blood of pregnant mice was collected and the serum was separated. The concentration of serum chorionic gonadotropin, placental lactogen, and estriol from pregnant mice was detected by radioimmunoassay （RIA）. Results Results showed that the concentration of serum chorionic gonadotropin decreased along with an increase in the days of pregnancy in both the experimental group and the normal control group. In the normal control group, however, the concentration increased slightly on the 18^th day of pregnancy. In contrast, the concentration of serum placental lactogen and estriol increased along with an increase in the days of pregnancy in both the experimental group and the normal control group. In the experimental group, however, the concentration decreased slightly on the 18^th day of pregnancy. For each group, the difference in the concentrations of serum chorionic gonadotropin, placental laetogen, and estriol from pregnant mice on the different days of pregnancy was statistically significant （CO： F normal control grouip=19. 306, Fexperimental group =57. 620; PL： Fnormal control group = 17. 449, Fexperimental group=46. 720; E3： Fnormal control group=239. 546, Fexperimental group =22. 841 ; P〈0.0l）. Results also showed that the concentrations of serum chorionic gonadotropin, placental lactogen and estriol in the experimental group were lower than those in the normal control group on the same days of pregnancy, though differences were statistically significant only on the 16^th and 18^th day （CG： t16=4.351, t18=13.367; PL： t16=4.034, t18=2.875; E3： t16=4.265, t18=11.4

关 键 词：刚地弓形虫 放射免疫分析法 绒毛膜促性腺激素 胎盘催乳素 雌三醇 

分 类 号：R382.5[医药卫生—医学寄生虫学]