C肽灌注对Wistar大鼠胰岛微循环的影响  

Effect of C peptide on pancreatic islet microcirculation in Wistar rats

作　　者：吴琳[1] 黄镇[2] Ake Sjoholm 高鑫[2] 

机构地区：[1]复旦大学附属中山医院老年病科,上海200032 [2]复旦大学附属中山医院内分泌科,上海200032 [3]Department of Clinincal Science and Education, Sodersjukhuset, Karolinska Institutet, Stockholm, Sweden

出　　处：《中华内分泌代谢杂志》2009年第5期535-538,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的测定生理剂量重组人C肽静脉注射对雄性Wistar大鼠胰岛微循环、血糖和胰岛素分泌的影响。方法成年雄性Wistar大鼠分为C肽组和对照组，分别给予C肽（75nmol／kg体重）和相同容积生理盐水静脉注射；每组再分为葡萄糖组和生理盐水组两个亚组。血流测定采用微球技术。结果C肽显著增高Wistar大鼠基础胰岛血流[C肽组vs对照组：（0．156±0．018vs0．050±0．003）ml·min^-1·g^-1胰腺，P〈0．01]和胰岛／胰腺血流比值[C肽组US对照组：（14．33±0．53vs9．16±0．64）％，P〈0．01]，胰腺血流向胰岛内重分布。C肽对糖负荷后胰岛微循环无显著影响。C肽降低大鼠腹腔糖耐量试验30min血糖[C肽组vs对照组：（7．9±0．7vs11．5±0．6）mmol／L，P〈0．01]；增加胰岛素分泌（AUC胰岛素（0-120），C肽组vs对照组：141．36±15．00vs49．78±5．60，P〈0．01）.结论C肽静脉注射改善Wistar大鼠基础胰岛微循环，调节糖负荷后胰岛素分泌。Objective To evaluate the effects of physiological doses of C-peptide on islet microcirculation, blood glucose, and insulin concentrations. Methods Adult male Wistar rats were divided into two groups i. v either with C-peptide （75nmoL/kg body wt）or saline （control）. Each group was further divided into 2 subgroups using 1 ml saline or 30% glucose. A microsphere technique was adopted to measure the pancreatic islet microcirculation. Results Intravenous injection of C-peptide markedly increased basal islet blood flow[ （0. 156± 0. 018 vs 0. 050±0. 003 ）ml·min^-1·g^-1 pancreas, P〈0.01 ] and fraction islet blood flow out of pancreatic blood flow[ （ 14.33±0.53 vs 9.16±0.64）% ,P〈0.01 ] ,but did not affect islet mierocirculation after intravenous glucose administration. In IPGTT,C-peptide decreased blood glucose at 30 min [ （7.9±0.7 vs 11.5±0.6） mmol/L,P〈 0.01 ] after glucose challenge and stimulated insulin secretion（ for AUCins（0-120）, 141.36±15.00 vs 49.78±5.60, P 〈 0. 01 ）. Conclusion C-peptide induces redistribution of pancreatic blood flow in favour of islet in nonnoglycemic rats,and modulates insulin release in hyperglyeaemic rats.

关 键 词：C肽 微球技术 胰岛微循环 腹腔糖耐量试验 

分 类 号：R587.1[医药卫生—内分泌]