静脉注射^(53)Cr标记吡啶甲酸铬对仔猪肝细胞DNA的影响  

The Effects of ^(53)Cr-chromium Picolinate Injected in Vein on Piglet Hepatocytes DNA

作　　者：郭亮[1,2] 张敏红[1] 郑姗姗[1] 冯京海[1] 谢鹏[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]天津市畜牧兽医研究所,天津300112

出　　处：《畜牧兽医学报》2009年第10期1487-1493,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30571350)

摘　　要：旨在研究静脉注射不同水平53Cr标记吡啶甲酸铬(53Cr(pic)3)对仔猪肝细胞DNA的影响。选取体质量(15.0±1.0)kg、健康的瘦肉型三元杂交(杜×长×大)公猪30头,按体质量、遗传背景相近的原则随机分成5组,每组6头,单笼个体饲养。每天早08:00-10:00分别对各组猪进行静脉注射,Ⅰ(对照)、Ⅱ、Ⅲ、Ⅳ、Ⅴ组分别注射0、8、200、400和800μg Cr.d-1用53Cr标记的吡啶甲酸铬,试验期14 d。检测指标有肝脏中抗氧化酶活性、尿中8-羟基脱氧鸟苷(8-OHdG)浓度、肝组织中示踪剂铬的浓度及DNA链断裂(彗星试验)情况。随着静脉注射吡啶甲酸铬剂量的升高,肝脏中抗氧化酶活性、尿液中8-OHdG的水平均未发生显著变化(P>0.05);吡啶甲酸铬在肝脏中的蓄积量随着静脉注射吡啶甲酸铬水平的升高而增加,且在200μg以上达到显著(P<0.05);在肝细胞彗星形状指标上,除8和200μg组彗星尾长显著低于对照组(P<0.05)外,各处理组彗星形状指标与对照组比较,差异均不显著(P>0.05),但400和800μg组各项彗星复合指标显著高于8μg组(P<0.05);静脉注射吡啶甲酸14 d,800μg Cr.d-1剂量范围内,仔猪肝脏抗氧化酶活性未发生显著变化,肝细胞DNA未受到氧化损伤;但吡啶甲酸铬在肝组织的蓄积量显著升高,且肝细胞DNA完整性与肝铬蓄积量之间存在显著量效关系。The objective of this study was to evaluate the effects of different level ^53 Cr（pic）3 injected in vein on piglet hepatocytes DNA. Thirty （15. 0 ± 1. 0） kg body weight male crossbred （D × L × Y） piglets were divided into five treatments with six replication each treatment, each was raised in one cage. In the 14 days experimental period, piglets in each treatment were respectively injected with 0,8,200,400,800 μg Cr.d^-1 at 08 ： 00-10 ： 00 every morning. The antioxidant enzymes activity in liver, the level of 8-OHdG in urine, the content of chromic in liver, and comet figure were measured. About the antioxidant enzymes activity in liver and the level of 8-OHdG in urine, no significant difference was detected among all groups （P〉0.05）. The content of chromium in liver was increased by increasing ^53Cr（pic）3. Compared to control, the concentration of chromium in liver was markedly increased while the chromium injected was more than 200μg Cr （^53Cr（pic）3） （P〈0. 05）. In comparison with control, no significant difference was discerned in comet figure, except the tail length of 8 and 200μg group decreased significantly （P〈0.05）. Compared to 8 μg group, the indicators of comet assay in 400 and 800μg group increased significantly （P〈0.05）. Chromium picolinate below 800 μg Cr.d^-1 injected in vein for 14 day, does not induce the change of antioxidant enzymes activity in liver and DNA oxidative damage, but the content of chromium in liver was increased with S3Cr（pic）3 increasing, it has dose-effect relation- ship between the content of chromium in liver and DNA integrity in hepatocytes of piglet.

关 键 词：53Cr标记吡啶甲酸铬 静脉注射 仔猪 DNA 

分 类 号：S828[农业科学—畜牧学] S816.72[农业科学—畜牧兽医]